Selection of a novel FGF23-binding peptide antagonizing the inhibitory effect of FGF23 on phosphate uptake
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6283-5 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6283-5 | ||
| 245 | 0 | 0 | |a Selection of a novel FGF23-binding peptide antagonizing the inhibitory effect of FGF23 on phosphate uptake |h [Elektronische Daten] |c [Tao Huang, Xiaomian Lin, Quchou Li, Wu Luo, Li Song, Xiangpeng Tan, Wenhui Wang, Xiaokun Li, Xiaoping Wu] |
| 520 | 3 | |a Fibroblast growth factor 23 (FGF23) is a bone-derived endocrine regulator of phosphate homeostasis and has been considered as a potential therapeutic target for hypophosphatemic disorders. Herein, we isolated a novel FGF23-binding peptide by screening a phage display library with FGF23180-205, the minimal epitope of FGF23 binding to the binary fibroblast growth factor receptor (FGFR)-Klotho complex. The corresponding peptide (referred to as 23-b6) showed high homology to the immunoglobulin-like (Ig-like) domain III (D3) of FGFR1c, the predominant receptor mediating the phosphaturic activity of FGF23. The 23-b6 peptide and panning target FGF23180-205 carried opposite charges and shared similar hydrophilic profiles. Functional analysis indicated that synthetic 23-b6 peptide exhibited antagonistic effect on the inhibition of phosphate uptake by FGF23 in opossum kidney cells (OK cells). The mechanisms of 23-b6 peptide impairing the bioactivity of FGF23 involved blockade of the activation of Erk cascade and up-regulation of NaPi-2a and NaPi-2c expression in OK cells. Our results demonstrate that the 23-b6 peptide is a potent FGF23 antagonist with increased effect on phosphate uptake in kidney cells and might have therapeutic potentials in hypophosphatemic disorders characterized by an abnormally high level of FGF23. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a Fibroblast growth factor 23 |2 nationallicence | |
| 690 | 7 | |a Phage display |2 nationallicence | |
| 690 | 7 | |a Phosphate uptake |2 nationallicence | |
| 690 | 7 | |a Hypophosphatemia |2 nationallicence | |
| 700 | 1 | |a Huang |D Tao |u Institute of Tissue Transplantation and Immunology, Jinan University, 510632, Guangzhou, China |4 aut | |
| 700 | 1 | |a Lin |D Xiaomian |u Institute of Tissue Transplantation and Immunology, Jinan University, 510632, Guangzhou, China |4 aut | |
| 700 | 1 | |a Li |D Quchou |u Institute of Tissue Transplantation and Immunology, Jinan University, 510632, Guangzhou, China |4 aut | |
| 700 | 1 | |a Luo |D Wu |u Institute of Tissue Transplantation and Immunology, Jinan University, 510632, Guangzhou, China |4 aut | |
| 700 | 1 | |a Song |D Li |u Institute of Tissue Transplantation and Immunology, Jinan University, 510632, Guangzhou, China |4 aut | |
| 700 | 1 | |a Tan |D Xiangpeng |u Institute of Tissue Transplantation and Immunology, Jinan University, 510632, Guangzhou, China |4 aut | |
| 700 | 1 | |a Wang |D Wenhui |u Institute of Tissue Transplantation and Immunology, Jinan University, 510632, Guangzhou, China |4 aut | |
| 700 | 1 | |a Li |D Xiaokun |u School of Pharmaceutical Science, Wenzhou Medical University, 325035, Wenzhou, China |4 aut | |
| 700 | 1 | |a Wu |D Xiaoping |u Institute of Tissue Transplantation and Immunology, Jinan University, 510632, Guangzhou, China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/7(2015-04-01), 3169-3177 |x 0175-7598 |q 99:7<3169 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6283-5 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6283-5 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Huang |D Tao |u Institute of Tissue Transplantation and Immunology, Jinan University, 510632, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Lin |D Xiaomian |u Institute of Tissue Transplantation and Immunology, Jinan University, 510632, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Li |D Quchou |u Institute of Tissue Transplantation and Immunology, Jinan University, 510632, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Luo |D Wu |u Institute of Tissue Transplantation and Immunology, Jinan University, 510632, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Song |D Li |u Institute of Tissue Transplantation and Immunology, Jinan University, 510632, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Tan |D Xiangpeng |u Institute of Tissue Transplantation and Immunology, Jinan University, 510632, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wang |D Wenhui |u Institute of Tissue Transplantation and Immunology, Jinan University, 510632, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Li |D Xiaokun |u School of Pharmaceutical Science, Wenzhou Medical University, 325035, Wenzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wu |D Xiaoping |u Institute of Tissue Transplantation and Immunology, Jinan University, 510632, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/7(2015-04-01), 3169-3177 |x 0175-7598 |q 99:7<3169 |1 2015 |2 99 |o 253 | ||