caa a22 4500 605500274 CHVBK 20210128100555.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00253-014-6365-4 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6365-4 Enhancing stress tolerance by overexpression of a methionine sulfoxide reductase A ( MsrA ) gene in Pleurotus ostreatus [Elektronische Daten] [Chaomin Yin, Liesheng Zheng, Jihong Zhu, Liguo Chen, Aimin Ma] Proteins are subjected to modification by reactive oxygen species (ROS), and oxidation of specific amino acid residues can impair their biological functions. Methionine as a sulfur-containing amino acid is easily oxidized to methionine sulfoxide (MetSO). The modified methionine can be repaired by methionine sulfoxide reductase (Msr), an enzyme that reverses oxidation of methionine in proteins. In this study, a methionine sulfoxide reductase A (PoMsrA) gene from Pleurotus ostreatus was cloned and characterized. Furthermore, the function of PoMsrA gene was analyzed by overexpression in P. ostreatus via Agrobacterium-mediated transformation. Stable integration of the target gene into the genome of P. ostreatus was confirmed by PCR, fluorescence observation, and Southern blot hybridization. qRT-PCR analysis showed that PoMsrA was highly expressed in the stage of mature and young fruiting bodies as well as the osmotic stress condition of 0.3M NaCl. Additionally, the transgenic strains with PoMsrA overexpression exhibited an enhanced tolerance to high temperature, high osmotic stress, and oxidative stress. This suggests that PoMsrA is an active player in the protection of the cellular proteins from oxidative stress damage. Springer-Verlag Berlin Heidelberg, 2015 Pleurotus ostreatus nationallicence Methionine sulfoxide reductase A nationallicence Protein oxidation nationallicence Overexpression nationallicence Oxidative and abiotic stresses nationallicence Yin Chaomin College of Food Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China aut Zheng Liesheng College of Plant Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China aut Zhu Jihong College of Food Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China aut Chen Liguo College of Plant Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China aut Ma Aimin College of Food Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 3115-3126 0175-7598 99:7<3115 2015 99 253 https://doi.org/10.1007/s00253-014-6365-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6365-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yin Chaomin College of Food Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Zheng Liesheng College of Plant Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Zhu Jihong College of Food Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Chen Liguo College of Plant Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 700 1- Ma Aimin College of Food Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 3115-3126 0175-7598 99:7<3115 2015 99 253