caa a22 4500 605500290 CHVBK 20210128100555.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00253-014-6213-6 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6213-6 Profile of bacterial communities in South African mine-water samples using Illumina next-generation sequencing platform [Elektronische Daten] [Jitendra Keshri, Boitumelo Mankazana, Maggy Momba] Mine water is an example of an extreme environment that contains a large number of diverse and specific bacteria. It is imperative to gain an understanding of these bacterial communities in order to develop effective strategies for the bioremediation of polluted aquatic systems. In this study, the high-throughput sequencing approach was used to characterize the bacterial communities in two different mine waters of South Africa: vanadium and gold mine water. Over 2629 operational taxonomic units (OTUs) were recovered from 15,802 reads of the 16S ribosomal RNA (rRNA) gene. They represented 8 phyla, 43 orders, 84 families and 105 genera. Proteobacteria and unclassified bacterial sequences were the most dominant. Apart from these, Firmicutes, Bacteroidetes, Actinobacteria, Candidate phylum OD1, Cyanobacteria, Verrucomicrobia and Deinococcus-Thermus were the recovered phyla, although their relative abundance differed between both the mine-water samples. Yet, diversity indices suggested that the bacterial communities inhabiting the vanadium mine water were more diverse than those in gold mine water. Interestingly, substantial percentages of the reads from either sample (58% in vanadium and 17% in gold mine water) could not be assigned to any phylum and remained unclassified, suggesting hitherto unidentified populations, and vast untapped microbial diversity. Overall, the results of this study exhibited bacterial community structures with high diversity in mine water, which can be explored further for their role in bioremediation and environmental management. Springer-Verlag Berlin Heidelberg, 2014 Gold mine nationallicence Vanadium mine nationallicence Community structure nationallicence Next-generation sequencing nationallicence Keshri Jitendra Department of Environmental, Water and Earth Sciences, Faculty of Science, Tshwane University of Technology, Pretoria, Gauteng, South Africa aut Mankazana Boitumelo Department of Environmental, Water and Earth Sciences, Faculty of Science, Tshwane University of Technology, Pretoria, Gauteng, South Africa aut Momba Maggy Department of Environmental, Water and Earth Sciences, Faculty of Science, Tshwane University of Technology, Pretoria, Gauteng, South Africa aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 3233-3242 0175-7598 99:7<3233 2015 99 253 https://doi.org/10.1007/s00253-014-6213-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6213-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Keshri Jitendra Department of Environmental, Water and Earth Sciences, Faculty of Science, Tshwane University of Technology, Pretoria, Gauteng, South Africa aut NATIONALLICENCE 700 1- Mankazana Boitumelo Department of Environmental, Water and Earth Sciences, Faculty of Science, Tshwane University of Technology, Pretoria, Gauteng, South Africa aut NATIONALLICENCE 700 1- Momba Maggy Department of Environmental, Water and Earth Sciences, Faculty of Science, Tshwane University of Technology, Pretoria, Gauteng, South Africa aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 3233-3242 0175-7598 99:7<3233 2015 99 253