caa a22 4500 605500312 CHVBK 20210128100555.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00253-015-6413-8 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6413-8 Application of recombinant Pediococcus acidilactici BD16 ( fcs + [Elektronische Daten] ech + ) in malolactic fermentation / [Baljinder Kaur, Balvir Kumar, Gaganjot Kaur, Debkumar Chakraborty, Kiranjeet Kaur] This study was conducted to enhance flavor characteristics of wine by malolactic fermentation using recombinant Pediococcus acidilactici BD16 (fcs +/ech +) encoding synthetic genes of feruloyl-CoA synthetase and enoyl-CoA hydratase. After malolactic fermentation, wine phenolics were characterized using LCMS-ESI technique and a significant improvement in the antioxidant activity and flavor characteristics of wine was observed due to increased concentration of cinnamic acid derivatives. This proof of concept study highlights the role of recombinant P. acidilactici BD16 (fcs +/ech +) in improving flavor as well as aroma of wine due to production of several phenolic derivatives during secondary fermentation. A novel metabolic pathway was predicted from mass spectral analysis data that indicates biotransformation of cinnamic acid and derivatives into apigenin, catechin, coniferyl aldehyde, cyanidin, hydroxybenzoic acids, laricitrin, luteolin, malvidin 3-glucoside, myricetin, naringenin, pelargonin, piceatannol, querecitin, and vanillin that not only increased the overall consumer appreciation but also improved nutritional and probably the therapeutic properties of wines. This is a first evidence-based study where role of recombinant P. acidilactici BD16 (fcs +/ech +) in the wine secondary fermentation has been elucidated. Springer-Verlag Berlin Heidelberg, 2015 Wine nationallicence Malolactic fermentation nationallicence Lactic acid bacteria nationallicence Pediococcus acidilactici nationallicence Phenolic compounds nationallicence Kaur Baljinder Department of Biotechnology, Punjabi University, Patiala, India aut Kumar Balvir Department of Biotechnology, Punjabi University, Patiala, India aut Kaur Gaganjot Department of Biotechnology, Punjabi University, Patiala, India aut Chakraborty Debkumar Department of Biotechnology, Punjabi University, Patiala, India aut Kaur Kiranjeet Department of Biotechnology, Punjabi University, Patiala, India aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 3015-3028 0175-7598 99:7<3015 2015 99 253 https://doi.org/10.1007/s00253-015-6413-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6413-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kaur Baljinder Department of Biotechnology, Punjabi University, Patiala, India aut NATIONALLICENCE 700 1- Kumar Balvir Department of Biotechnology, Punjabi University, Patiala, India aut NATIONALLICENCE 700 1- Kaur Gaganjot Department of Biotechnology, Punjabi University, Patiala, India aut NATIONALLICENCE 700 1- Chakraborty Debkumar Department of Biotechnology, Punjabi University, Patiala, India aut NATIONALLICENCE 700 1- Kaur Kiranjeet Department of Biotechnology, Punjabi University, Patiala, India aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 3015-3028 0175-7598 99:7<3015 2015 99 253