caa a22 4500 605500339 CHVBK 20210128100555.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00253-015-6432-5 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6432-5 Microbial communities in liquid and fiber fractions of food waste digestates are differentially resistant to inhibition by ammonia [Elektronische Daten] [Wei Peng, Fan Lü, Liming Shao, Pinjing He] The effect of different concentrations of ammonia (1.0-7.0g/L) during mesophilic anaerobic digestion with fiber or liquid digestate as inoculum was examined. Evolution of microbial community within fiber and liquid digestates was quantitatively assessed by the intact lipid analysis methods and qualitatively by DNA fingerprint methods in order to determine their resistance to ammonia inhibition. The results showed that an increased level of total ammonia nitrogen prolonged the lag phase of fiber digestates while reduced the metabolic rate of liquid digestates. Fiber digestates had 19.6-50.9-fold higher concentrations of phospholipid fatty acids (PLFA) compared to liquid digestates, whereas concentrations of phospholipid ether lipids (PLEL) in the fiber digestates were only 2.91-17.6-fold higher compared to liquid digestates. Although the cell concentration in liquid fraction was far lower than that in the fiber one, the ammonia-resistant ability and the methanization efficiency of the liquid digestate was superior to the fiber digestate. The bacterial profiles were affected more by the type of digestate inoculum compared to the concentration of ammonia. Principal component analysis indicated that the lipids technique was superior to the DNA technique for bacterial quantification but detected less archaeal diversity. Springer-Verlag Berlin Heidelberg, 2015 Anaerobic digestion nationallicence Ammonia inhibition nationallicence Intact polar lipid nationallicence Phospholipid fatty acid biomarker nationallicence Phospholipid ether lipid nationallicence Peng Wei State Key Laboratory of Pollution Control & Resource Reuse, Tongji University, 200092, Shanghai, People's Republic of China aut Lü Fan State Key Laboratory of Pollution Control & Resource Reuse, Tongji University, 200092, Shanghai, People's Republic of China aut Shao Liming Institute of Waste Treatment and Reclamation, Tongji University, 200092, Shanghai, People's Republic of China aut He Pinjing Institute of Waste Treatment and Reclamation, Tongji University, 200092, Shanghai, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 3317-3326 0175-7598 99:7<3317 2015 99 253 https://doi.org/10.1007/s00253-015-6432-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6432-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Peng Wei State Key Laboratory of Pollution Control & Resource Reuse, Tongji University, 200092, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Lü Fan State Key Laboratory of Pollution Control & Resource Reuse, Tongji University, 200092, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Shao Liming Institute of Waste Treatment and Reclamation, Tongji University, 200092, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- He Pinjing Institute of Waste Treatment and Reclamation, Tongji University, 200092, Shanghai, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 3317-3326 0175-7598 99:7<3317 2015 99 253