caa a22 4500 605500363 CHVBK 20210128100555.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00253-015-6377-8 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6377-8 Genomic and chemical insights into biosurfactant production by the mangrove-derived strain Bacillus safensis CCMA-560 [Elektronische Daten] [Daniela Domingos, Andreia de Faria, Renan de Souza Galaverna, Marcos Eberlin, Paul Greenfield, Tiago Zucchi, Itamar Melo, Nai Tran-Dinh, David Midgley, Valéria de Oliveira] Many Bacillus species can produce biosurfactant, although most of the studies on lipopeptide production by this genus have been focused on Bacillus subtilis. Surfactants are broadly used in pharmaceutical, food and petroleum industry, and biological surfactant shows some advantages over the chemical surfactants, such as less toxicity, production from renewable, cheaper feedstocks and development of novel recombinant hyperproducer strains. This study is aimed to unveil the biosurfactant metabolic pathway and chemical composition in Bacillus safensis strain CCMA-560. The whole genome of the CCMA-560 strain was previously sequenced, and with the aid of bioinformatics tools, its biosurfactant metabolic pathway was compared to other pathways of closely related species. Fourier transform infrared (FTIR) and high-resolution TOF mass spectrometry (MS) were used to characterize the biosurfactant molecule. B. safensis CCMA-560 metabolic pathway is similar to other Bacillus species; however, some differences in amino acid incorporation were observed, and chemical analyses corroborated the genetic results. The strain CCMA-560 harbours two genes flanked by srfAC and srfAD not present in other Bacillus spp., which can be involved in the production of the analogue gramicidin. FTIR and MS showed that B. safensis CCMA-560 produces a mixture of at least four lipopeptides with seven amino acids incorporated and a fatty acid chain with 14 carbons, which makes this molecule similar to the biosurfactant of Bacillus pumilus, namely, pumilacidin. This is the first report on the biosurfactant production by B. safensis, encompassing the investigation of the metabolic pathway and chemical characterization of the biosurfactant molecule. Springer-Verlag Berlin Heidelberg, 2015 Pumilacidin nationallicence Metabolic pathway nationallicence Chemical characterization nationallicence Surfactant nationallicence Bacillus nationallicence Domingos Daniela Microbial Resources Division, Research Center for Chemistry, Biology and Agriculture (CPQBA), University of Campinas (UNICAMP), Av. Alexandre Cazelatto, 999, 13148-218, Campinas, SP, Brazil aut de Faria Andreia Department of Chemical and Environmental Engineering, Yale University, 06520-8286, New Haven, CT, USA aut de Souza Galaverna Renan ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry, University of Campinas (UNICAMP), Campinas, SP, Brazil aut Eberlin Marcos ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry, University of Campinas (UNICAMP), Campinas, SP, Brazil aut Greenfield Paul CSIRO Mathematics, Informatics and Statistics, 2113, North Ryde, NSW, Australia aut Zucchi Tiago Laboratory of Environmental Microbiology, EMBRAPA Environment, Jaguariúna, São Paulo, Brazil aut Melo Itamar Laboratory of Environmental Microbiology, EMBRAPA Environment, Jaguariúna, São Paulo, Brazil aut Tran-Dinh Nai CSIRO Animal, Food and Health Sciences, 2113, North Ryde, NSW, Australia aut Midgley David CSIRO Animal, Food and Health Sciences, 2113, North Ryde, NSW, Australia aut de Oliveira Valéria Microbial Resources Division, Research Center for Chemistry, Biology and Agriculture (CPQBA), University of Campinas (UNICAMP), Av. Alexandre Cazelatto, 999, 13148-218, Campinas, SP, Brazil aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 3155-3167 0175-7598 99:7<3155 2015 99 253 https://doi.org/10.1007/s00253-015-6377-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6377-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Domingos Daniela Microbial Resources Division, Research Center for Chemistry, Biology and Agriculture (CPQBA), University of Campinas (UNICAMP), Av. Alexandre Cazelatto, 999, 13148-218, Campinas, SP, Brazil aut NATIONALLICENCE 700 1- de Faria Andreia Department of Chemical and Environmental Engineering, Yale University, 06520-8286, New Haven, CT, USA aut NATIONALLICENCE 700 1- de Souza Galaverna Renan ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry, University of Campinas (UNICAMP), Campinas, SP, Brazil aut NATIONALLICENCE 700 1- Eberlin Marcos ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry, University of Campinas (UNICAMP), Campinas, SP, Brazil aut NATIONALLICENCE 700 1- Greenfield Paul CSIRO Mathematics, Informatics and Statistics, 2113, North Ryde, NSW, Australia aut NATIONALLICENCE 700 1- Zucchi Tiago Laboratory of Environmental Microbiology, EMBRAPA Environment, Jaguariúna, São Paulo, Brazil aut NATIONALLICENCE 700 1- Melo Itamar Laboratory of Environmental Microbiology, EMBRAPA Environment, Jaguariúna, São Paulo, Brazil aut NATIONALLICENCE 700 1- Tran-Dinh Nai CSIRO Animal, Food and Health Sciences, 2113, North Ryde, NSW, Australia aut NATIONALLICENCE 700 1- Midgley David CSIRO Animal, Food and Health Sciences, 2113, North Ryde, NSW, Australia aut NATIONALLICENCE 700 1- de Oliveira Valéria Microbial Resources Division, Research Center for Chemistry, Biology and Agriculture (CPQBA), University of Campinas (UNICAMP), Av. Alexandre Cazelatto, 999, 13148-218, Campinas, SP, Brazil aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 3155-3167 0175-7598 99:7<3155 2015 99 253