caa a22 4500 605500398 CHVBK 20210128100555.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00253-014-6294-2 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6294-2 Isolation and characterization of a gamma-aminobutyric acid producing strain Lactobacillus buchneri WPZ001 that could efficiently utilize xylose and corncob hydrolysate [Elektronische Daten] [Anqi Zhao, Xiaoqing Hu, Lu Pan, Xiaoyuan Wang] Lactobacillus buchneri strain WPZ001 that could efficiently produce gamma-aminobutyric acid was isolated from Chinese fermented sausages. Optimal cultivation conditions for gamma-aminobutyric acid production in L. buchneri WPZ001 were determined, and xylose was found to be the best carbon source. Using xylose as the sole carbon source, 70g/L gamma-aminobutyric acid was produced by flask fermentation of L. buchneri WPZ001 for 48h, and the harvested cells could continue to convert monosodium glutamate to gamma-aminobutyric acid in buffer and produce 59g gamma-aminobutyric acid after eight runs of biotransformation; the total yield of gamma-aminobutyric acid reached 129g/L. This combination strategy also worked well when the low-cost corncob hydrolysate was used as the sole carbon source, and the yield of gamma-aminobutyric acid reached 117g/L. The results indicate that L. buchneri WPZ001 has great potential for industrial production of gamma-aminobutyric acid. Springer-Verlag Berlin Heidelberg, 2014 Lactobacillus buchneri nationallicence Gamma-aminobutyric acid nationallicence Xylose nationallicence Corncob hydrolysate nationallicence Zhao Anqi State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China aut Hu Xiaoqing Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi, China aut Pan Lu Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi, China aut Wang Xiaoyuan State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 3191-3200 0175-7598 99:7<3191 2015 99 253 https://doi.org/10.1007/s00253-014-6294-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6294-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhao Anqi State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China aut NATIONALLICENCE 700 1- Hu Xiaoqing Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi, China aut NATIONALLICENCE 700 1- Pan Lu Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi, China aut NATIONALLICENCE 700 1- Wang Xiaoyuan State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 3191-3200 0175-7598 99:7<3191 2015 99 253