caa a22 4500 605500401 CHVBK 20210128100555.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00253-014-6235-0 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6235-0 Effect of sulfate addition on methane production and sulfate reduction in a mesophilic acetate-fed anaerobic reactor [Elektronische Daten] [Sen-Lin Yang, Yue-Qin Tang, Min Gou, Xia Jiang] A mesophilic anaerobic moving bed biofilm reactor (MBBR) was operated to evaluate the effect of sulfate addition on methane production and sulfate reduction using acetate as the sole carbon source. The results show that at the organic loading rate of 4.0g TOC/L/day, the TOC removal efficiencies and the biogas production rates achieved over 95% and 7000mL/L/day without sulfate, respectively, and slightly decreased with sulfate addition (500-800mg/L). Methane production capacities were not influenced significantly with the addition of sulfate, while sulfate reduction efficiencies were not stable with 23-87% in the acetate-fed reactor. Fluorescent in situ hybridization (FISH) was used to analyze the functional microbial compositions of acetate-degrading methane-producing bacteria (MPB) and sulfate-reducing bacteria (SRB) in the reactor. The results found that as the increase of sulfate concentration, the proportion of Methanomicrobiales increased up to 58 ± 2%, while Methanosaeta and Methanosarcina decreased. The dominant methanogens shifted into hydrogenotrophic methanogens from even distribution of acetoclastic and hydrogenotrophic methanogens. When hydrogenotrophic methanogens were dominant, sulfate reduction efficiency was high, while sulfate reduction efficiency was low as acetoclastic methanogens were dominant. Springer-Verlag Berlin Heidelberg, 2014 Acetate degradation nationallicence Anaerobic digestion nationallicence Methane production nationallicence Sulfate reduction nationallicence Yang Sen-Lin College of Architecture and Environment, Sichuan University, 610065, Chengdu, People's Republic of China aut Tang Yue-Qin College of Architecture and Environment, Sichuan University, 610065, Chengdu, People's Republic of China aut Gou Min College of Architecture and Environment, Sichuan University, 610065, Chengdu, People's Republic of China aut Jiang Xia College of Architecture and Environment, Sichuan University, 610065, Chengdu, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 3269-3277 0175-7598 99:7<3269 2015 99 253 https://doi.org/10.1007/s00253-014-6235-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6235-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Sen-Lin College of Architecture and Environment, Sichuan University, 610065, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Tang Yue-Qin College of Architecture and Environment, Sichuan University, 610065, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Gou Min College of Architecture and Environment, Sichuan University, 610065, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Jiang Xia College of Architecture and Environment, Sichuan University, 610065, Chengdu, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/7(2015-04-01), 3269-3277 0175-7598 99:7<3269 2015 99 253