Improving furfural tolerance of Zymomonas mobilis by rewiring a sigma factor RpoD protein
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6577-2 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6577-2 | ||
| 245 | 0 | 0 | |a Improving furfural tolerance of Zymomonas mobilis by rewiring a sigma factor RpoD protein |h [Elektronische Daten] |c [Fu-Rong Tan, Li-Chun Dai, Bo Wu, Han Qin, Zong-Xia Shui, Jing-Li Wang, Qi-Li Zhu, Qi-Chun Hu, Zhi-Yong Ruan, Ming-Xiong He] |
| 520 | 3 | |a Furfural from lignocellulosic hydrolysates is the key inhibitor for bio-ethanol fermentation. In this study, we report a strategy of improving the furfural tolerance in Zymomonas mobilis on the transcriptional level by engineering its global transcription sigma factor (σ70, RpoD) protein. Three furfural tolerance RpoD mutants (ZM4-MF1, ZM4-MF2, and ZM4-MF3) were identified from error-prone PCR libraries. The best furfural-tolerance strain ZM4-MF2 reached to the maximal cell density (OD600) about 2.0 after approximately 30h, while control strain ZM4-rpoD reached its highest cell density of about 1.3 under the same conditions. ZM4-MF2 also consumed glucose faster and yield higher ethanol; expression levels and key Entner-Doudoroff (ED) pathway enzymatic activities were also compared to control strain under furfural stress condition. Our results suggest that global transcription machinery engineering could potentially be used to improve stress tolerance and ethanol production in Z. mobilis. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Furfural tolerance |2 nationallicence | |
| 690 | 7 | |a Ethanol production |2 nationallicence | |
| 690 | 7 | |a Global transcription machinery engineering |2 nationallicence | |
| 690 | 7 | |a Error-prone PCR |2 nationallicence | |
| 690 | 7 | |a Sigma factor |2 nationallicence | |
| 690 | 7 | |a RpoD protein |2 nationallicence | |
| 700 | 1 | |a Tan |D Fu-Rong |u Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China |4 aut | |
| 700 | 1 | |a Dai |D Li-Chun |u Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China |4 aut | |
| 700 | 1 | |a Wu |D Bo |u Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China |4 aut | |
| 700 | 1 | |a Qin |D Han |u Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China |4 aut | |
| 700 | 1 | |a Shui |D Zong-Xia |u Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China |4 aut | |
| 700 | 1 | |a Wang |D Jing-Li |u Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China |4 aut | |
| 700 | 1 | |a Zhu |D Qi-Li |u Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China |4 aut | |
| 700 | 1 | |a Hu |D Qi-Chun |u Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China |4 aut | |
| 700 | 1 | |a Ruan |D Zhi-Yong |u Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, 100081, Beijing, People's Republic of China |4 aut | |
| 700 | 1 | |a He |D Ming-Xiong |u Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/12(2015-06-01), 5363-5371 |x 0175-7598 |q 99:12<5363 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6577-2 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6577-2 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Tan |D Fu-Rong |u Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Dai |D Li-Chun |u Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wu |D Bo |u Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Qin |D Han |u Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Shui |D Zong-Xia |u Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wang |D Jing-Li |u Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhu |D Qi-Li |u Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Hu |D Qi-Chun |u Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ruan |D Zhi-Yong |u Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, 100081, Beijing, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a He |D Ming-Xiong |u Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/12(2015-06-01), 5363-5371 |x 0175-7598 |q 99:12<5363 |1 2015 |2 99 |o 253 | ||