caa a22 4500 605500428 CHVBK 20210128100555.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00253-015-6577-2 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6577-2 Improving furfural tolerance of Zymomonas mobilis by rewiring a sigma factor RpoD protein [Elektronische Daten] [Fu-Rong Tan, Li-Chun Dai, Bo Wu, Han Qin, Zong-Xia Shui, Jing-Li Wang, Qi-Li Zhu, Qi-Chun Hu, Zhi-Yong Ruan, Ming-Xiong He] Furfural from lignocellulosic hydrolysates is the key inhibitor for bio-ethanol fermentation. In this study, we report a strategy of improving the furfural tolerance in Zymomonas mobilis on the transcriptional level by engineering its global transcription sigma factor (σ70, RpoD) protein. Three furfural tolerance RpoD mutants (ZM4-MF1, ZM4-MF2, and ZM4-MF3) were identified from error-prone PCR libraries. The best furfural-tolerance strain ZM4-MF2 reached to the maximal cell density (OD600) about 2.0 after approximately 30h, while control strain ZM4-rpoD reached its highest cell density of about 1.3 under the same conditions. ZM4-MF2 also consumed glucose faster and yield higher ethanol; expression levels and key Entner-Doudoroff (ED) pathway enzymatic activities were also compared to control strain under furfural stress condition. Our results suggest that global transcription machinery engineering could potentially be used to improve stress tolerance and ethanol production in Z. mobilis. Springer-Verlag Berlin Heidelberg, 2015 Furfural tolerance nationallicence Ethanol production nationallicence Global transcription machinery engineering nationallicence Error-prone PCR nationallicence Sigma factor nationallicence RpoD protein nationallicence Tan Fu-Rong Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut Dai Li-Chun Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut Wu Bo Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut Qin Han Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut Shui Zong-Xia Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut Wang Jing-Li Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut Zhu Qi-Li Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut Hu Qi-Chun Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut Ruan Zhi-Yong Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, 100081, Beijing, People's Republic of China aut He Ming-Xiong Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/12(2015-06-01), 5363-5371 0175-7598 99:12<5363 2015 99 253 https://doi.org/10.1007/s00253-015-6577-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6577-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tan Fu-Rong Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Dai Li-Chun Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Wu Bo Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Qin Han Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Shui Zong-Xia Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Wang Jing-Li Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Zhu Qi-Li Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Hu Qi-Chun Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 700 1- Ruan Zhi-Yong Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, 100081, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- He Ming-Xiong Biogas Institute of Ministry of Agriculture, Biomass Energy Technology Research Centre, Section 4-13, Renming Nanlu, 610041, Chengdu, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/12(2015-06-01), 5363-5371 0175-7598 99:12<5363 2015 99 253