Construction of a novel phenol synthetic pathway in Escherichia coli through 4-hydroxybenzoate decarboxylation
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6497-1 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6497-1 | ||
| 245 | 0 | 0 | |a Construction of a novel phenol synthetic pathway in Escherichia coli through 4-hydroxybenzoate decarboxylation |h [Elektronische Daten] |c [Liangtian Miao, Qingyan Li, Aipo Diao, Xueli Zhang, Yanhe Ma] |
| 520 | 3 | |a Phenol is a bulk chemical with lots of applications in the chemical industry. Fermentative production of phenol had been realized in both Pseudomonas putida and Escherichia coli by recruiting tyrosine phenol-lyase (TPL). The TPL pathway needs tyrosine as the direct precursor for phenol production. In this work, a novel phenol synthetic pathway was created in E. coli by recruiting 4-hydroxybenzoate decarboxylase, which can convert 4-hydroxybenzoate to phenol and carbon dioxide. Activating 3-deoxy-d-arabino-heptulosonate-7-phosphate (DAHP) synthase and chorismate pyruvate lyase (UbiC) through plasmid overexpression led to 7- and 69-fold increase of phenol production, respectively, demonstrating that these two enzymes were the rate-limiting steps for phenol production. Genetically stable strains were then obtained by gene integration and gene modulation directly in chromosome. Phenol titer increased 147-fold (from 1.7 to 250mg/L) after modulating the DAHP synthase, UbiC, and 4-hydroxybenzoate decarboxylase genes in chromosome. Five solvents were tested for two-phase extractive fermentation to eliminate phenol toxicity to E. coli cells. Tributyrin and dibutyl phthalate were the best two solvents for improving phenol production, leading to 23 and 30% increase of total phenol production, respectively. Two-phase fed-batch fermentation of the best strain Phe009 was performed in a 7L fermentor, which produced 9.51g/L phenol with a yield of 0.061g/g glucose. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Phenol |2 nationallicence | |
| 690 | 7 | |a 4-hydroxybenzoate decarboxylase |2 nationallicence | |
| 690 | 7 | |a Two-phase extractive fermentation |2 nationallicence | |
| 690 | 7 | |a Escherichia coli |2 nationallicence | |
| 700 | 1 | |a Miao |D Liangtian |u Tianjin University of Science & Technology, 300457, Tianjin, China |4 aut | |
| 700 | 1 | |a Li |D Qingyan |u Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 300308, Tianjin, China |4 aut | |
| 700 | 1 | |a Diao |D Aipo |u Tianjin University of Science & Technology, 300457, Tianjin, China |4 aut | |
| 700 | 1 | |a Zhang |D Xueli |u Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 300308, Tianjin, China |4 aut | |
| 700 | 1 | |a Ma |D Yanhe |u Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 300308, Tianjin, China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/12(2015-06-01), 5163-5173 |x 0175-7598 |q 99:12<5163 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6497-1 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6497-1 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Miao |D Liangtian |u Tianjin University of Science & Technology, 300457, Tianjin, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Li |D Qingyan |u Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 300308, Tianjin, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Diao |D Aipo |u Tianjin University of Science & Technology, 300457, Tianjin, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhang |D Xueli |u Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 300308, Tianjin, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ma |D Yanhe |u Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 300308, Tianjin, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/12(2015-06-01), 5163-5173 |x 0175-7598 |q 99:12<5163 |1 2015 |2 99 |o 253 | ||