caa a22 4500 605500444 CHVBK 20210128100556.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00253-015-6442-3 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6442-3 Metabolic engineering of Klebsiella pneumoniae for the production of cis , cis -muconic acid [Elektronische Daten] [Hwi-Min Jung, Moo-Young Jung, Min-Kyu Oh] cis,cis-Muconic acid (ccMA), a metabolic intermediate of Klebsiella pneumoniae, can be converted to adipic acid and terephthalic acid, which are important monomers of synthetic polymers. However, wild-type K. pneumoniae does not produce ccMA because intracellular carbon flow does not favor ccMA biosynthesis. In this study, several metabolic engineering strategies were used in an attempt to modify the wild-type strain to induce it to produce ccMA. First, by blocking the synthesis of aromatic amino acids, 343mg/L of catechol, a precursor of ccMA, was produced. Then, the native catechol 1,2-dioxygenasegene (catA) was overexpressed, which caused the strain to convert the catechol to ccMA. The production of ccMA was further improved by deletion of the muconate cycloisomerase gene (catB) and by deleting a feedback inhibitor of the aromatic amino acid pathway. Further improvement was achieved by adjusting the pH of the culture broth. The developed strain produced 2.1g/L of ccMA in flask cultivation. The results showed the potential of K. pneumoniae as a ccMA producer. Springer-Verlag Berlin Heidelberg, 2015 cis , cis -Muconic acid nationallicence Klebsiella pneumoniae nationallicence Aromatic amino acid pathway nationallicence Benzoate degradation pathway nationallicence Jung Hwi-Min Department of Chemical and Biological Engineering, Korea University, 136-713, Seoul, Korea aut Jung Moo-Young Department of Chemical and Biological Engineering, Korea University, 136-713, Seoul, Korea aut Oh Min-Kyu Department of Chemical and Biological Engineering, Korea University, 136-713, Seoul, Korea aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/12(2015-06-01), 5217-5225 0175-7598 99:12<5217 2015 99 253 https://doi.org/10.1007/s00253-015-6442-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6442-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jung Hwi-Min Department of Chemical and Biological Engineering, Korea University, 136-713, Seoul, Korea aut NATIONALLICENCE 700 1- Jung Moo-Young Department of Chemical and Biological Engineering, Korea University, 136-713, Seoul, Korea aut NATIONALLICENCE 700 1- Oh Min-Kyu Department of Chemical and Biological Engineering, Korea University, 136-713, Seoul, Korea aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/12(2015-06-01), 5217-5225 0175-7598 99:12<5217 2015 99 253