caa a22 4500 605500509 CHVBK 20210128100556.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00253-015-6402-y doi (NATIONALLICENCE)springer-10.1007/s00253-015-6402-y Characterization of the bacterial community involved in the bioflocculation process of wastewater organic matter in high-loaded MBRs [Elektronische Daten] [L. Faust, M. Szendy, C. Plugge, P. van den Brink, H. Temmink, H. Rijnaarts] High-loaded membrane bioreactors (HL-MBRs), i.e., bioreactors equipped with a membrane for biomass retention and operated at extremely short sludge and hydraulic retention times, can concentrate sewage organic matter to facilitate subsequent energy and chemical recovery from these organics. Bioflocculation, accomplished by microorganisms that produce extracellular polymers, is a very important mechanism in these reactors. Bacterial diversity of the sludge and supernatant fraction of HL-MBRs operated at very short sludge retention times (0.125, 0.5, and 1day) were determined using a PCR-denaturing gradient gel electrophoresis (DGGE) and clone library approach and compared to the diversity in sewage. Already at a sludge retention time (SRT) of 0.125day, a distinct bacterial community developed compared to the community in sewage. Bioflocculation, however, was low and the majority of the bacteria, especially Arcobacter, were present in the supernatant fraction. Upon increasing SRT from 0.125 to 1day, a much stronger bioflocculation was accompanied by an increased abundance of Bacteroidetes in the (solid) sludge fraction: 27.5% at an SRT of 0.5day and 46.4% at an SRT of 1day. Furthermore, cluster analysis of DGGE profiles revealed that the bacterial community structure in the sludge was different from that in the supernatant. To localize specific bacterial classes in the sludge flocs, fluorescence in situ hybridization (FISH) was carried out with three different bacterial probes. This showed that Betaproteobacteria formed clusters in the sludge flocs whereas Alphaproteobacteria and Gammaproteobacteria were mainly present as single cells. Springer-Verlag Berlin Heidelberg, 2015 Bioflocculation nationallicence Bacterial community nationallicence Membrane bioreactor nationallicence Wastewater treatment nationallicence Faust L. Wetsus-Centre of Excellence for Sustainable Water Technology, P.O. Box 1113, 8900 CC, Leeuwarden, The Netherlands aut Szendy M. Campus Essen-Biofilm Centre, Universität Duisburg-Essen, Universitätsstr. 5, 45141, Essen, Germany aut Plugge C. Wetsus-Centre of Excellence for Sustainable Water Technology, P.O. Box 1113, 8900 CC, Leeuwarden, The Netherlands aut van den Brink P. Wetsus-Centre of Excellence for Sustainable Water Technology, P.O. Box 1113, 8900 CC, Leeuwarden, The Netherlands aut Temmink H. Wetsus-Centre of Excellence for Sustainable Water Technology, P.O. Box 1113, 8900 CC, Leeuwarden, The Netherlands aut Rijnaarts H. Sub-department of Environmental Technology, Wageningen University, P.O. Box 8129, 6700 EV, Wageningen, The Netherlands aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/12(2015-06-01), 5327-5337 0175-7598 99:12<5327 2015 99 253 https://doi.org/10.1007/s00253-015-6402-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6402-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Faust L. Wetsus-Centre of Excellence for Sustainable Water Technology, P.O. Box 1113, 8900 CC, Leeuwarden, The Netherlands aut NATIONALLICENCE 700 1- Szendy M. Campus Essen-Biofilm Centre, Universität Duisburg-Essen, Universitätsstr. 5, 45141, Essen, Germany aut NATIONALLICENCE 700 1- Plugge C. Wetsus-Centre of Excellence for Sustainable Water Technology, P.O. Box 1113, 8900 CC, Leeuwarden, The Netherlands aut NATIONALLICENCE 700 1- van den Brink P. Wetsus-Centre of Excellence for Sustainable Water Technology, P.O. Box 1113, 8900 CC, Leeuwarden, The Netherlands aut NATIONALLICENCE 700 1- Temmink H. Wetsus-Centre of Excellence for Sustainable Water Technology, P.O. Box 1113, 8900 CC, Leeuwarden, The Netherlands aut NATIONALLICENCE 700 1- Rijnaarts H. Sub-department of Environmental Technology, Wageningen University, P.O. Box 8129, 6700 EV, Wageningen, The Netherlands aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/12(2015-06-01), 5327-5337 0175-7598 99:12<5327 2015 99 253