caa a22 4500 605500525 CHVBK 20210128100556.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00253-015-6556-7 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6556-7 Pull-in urea cycle for the production of fumaric acid in Escherichia coli [Elektronische Daten] [Ting Zhang, Zening Wang, Li Deng, Tianwei Tan, Fang Wang, Yajun Yan] Fumaric acid (FA) is an important raw material in the chemical and pharmaceutical industries. In this work, Escherichia coli was metabolically engineered for the production of FA. The fumA, fumB, fumC, and frdABCD genes were deleted to cut off the downstream pathway of FA. In addition, the iclR and arcA genes were also deleted to activate the glyoxylate shunt and to reinforce the oxidative Krebs cycle. To increase the FA yield, this base strain was further engineered to be pulled in the urea cycle by overexpressing the native carAB, argI, and heterologous rocF genes. The metabolites and the proteins of the Krebs cycle and the urea cycle were analyzed to confirm that the induced urea cycle improved the FA accumulation. With the induced urea cycle, the resulting strain ABCDIA-RAC was able to produce 11.38mmol/L of FA from 83.33mmol/L of glucose in a flask culture during 24h of incubation. Springer-Verlag Berlin Heidelberg, 2015 Fumaric acid nationallicence Escherichia coli nationallicence Urea cycle nationallicence Metabolic analysis nationallicence Zhang Ting Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, 100029, Beijing, People's Republic of China aut Wang Zening Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, 100029, Beijing, People's Republic of China aut Deng Li Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, 100029, Beijing, People's Republic of China aut Tan Tianwei Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, 100029, Beijing, People's Republic of China aut Wang Fang Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, 100029, Beijing, People's Republic of China aut Yan Yajun Biochemical Engineering Program, College of Engineering, University of Georgia, 30602, Athens, GA, USA aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/12(2015-06-01), 5033-5044 0175-7598 99:12<5033 2015 99 253 https://doi.org/10.1007/s00253-015-6556-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6556-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Ting Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, 100029, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Wang Zening Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, 100029, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Deng Li Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, 100029, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Tan Tianwei Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, 100029, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Wang Fang Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, 100029, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Yan Yajun Biochemical Engineering Program, College of Engineering, University of Georgia, 30602, Athens, GA, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/12(2015-06-01), 5033-5044 0175-7598 99:12<5033 2015 99 253