caa a22 4500 605500533 CHVBK 20210128100556.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00253-014-6317-z doi (NATIONALLICENCE)springer-10.1007/s00253-014-6317-z Surfactin from Bacillus subtilis displays an unexpected anti- Legionella activity [Elektronische Daten] [Clémence Loiseau, Margot Schlusselhuber, Renaud Bigot, Joanne Bertaux, Jean-Marc Berjeaud, Julien Verdon] A contaminant bacterial strain was found to exhibit an antagonistic activity against Legionella pneumophila, the causative agent of Legionnaires' disease. The bacterial strain was identified as a Bacillus subtilis and named B. subtilis AM1. PCR analysis revealed the presence of the sfp gene, involved in the biosynthesis of surfactin, a lipopeptide with versatile bioactive properties. The bioactive substances were extracted from AM1 cell-free supernatant with ethyl acetate and purified using reversed phase HPLC (RP-HPLC). Subsequent ESI-MS analyses indicated the presence of two active substances with protonated molecular ions at m/z 1008 and 1036Da, corresponding to surfactin isoforms. Structures of lipopeptides were further determined by tandem mass spectrometry and compared to the spectra of a commercially available surfactin mixture. Surfactin displays an antibacterial spectrum almost restricted to the Legionella genus (MICs range 1-4μg/mL) and also exhibits a weak activity toward the amoeba Acanthamoeba castellanii, known to be the natural reservoir of L. pneumophila. Anti-biofilm assays demonstrated that 66μg/mL of surfactin successfully eliminated 90% of a 6-day-old biofilm. In conclusion, this study reveals for the first time the potent activity of surfactin against Legionella sp. and preformed biofilms thus providing new directions toward the use and the development of lipopeptides for the control of Legionella spread in the environment. Springer-Verlag Berlin Heidelberg, 2015 Lipopeptides nationallicence Biosurfactants nationallicence Surfactin nationallicence Legionella nationallicence Bacillus nationallicence Biofilms nationallicence Loiseau Clémence Equipe Microbiologie de l'Eau, Université de Poitiers, Ecologie & Biologie des Interactions, UMR CNRS 7267, 1 Rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 9, France aut Schlusselhuber Margot Equipe Microbiologie de l'Eau, Université de Poitiers, Ecologie & Biologie des Interactions, UMR CNRS 7267, 1 Rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 9, France aut Bigot Renaud Equipe Microbiologie de l'Eau, Université de Poitiers, Ecologie & Biologie des Interactions, UMR CNRS 7267, 1 Rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 9, France aut Bertaux Joanne Equipe Ecologie Evolution Symbiose, Université de Poitiers, Ecologie & Biologie des Interactions, UMR CNRS 7267, 1 Rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 9, France aut Berjeaud Jean-Marc Equipe Microbiologie de l'Eau, Université de Poitiers, Ecologie & Biologie des Interactions, UMR CNRS 7267, 1 Rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 9, France aut Verdon Julien Equipe Microbiologie de l'Eau, Université de Poitiers, Ecologie & Biologie des Interactions, UMR CNRS 7267, 1 Rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 9, France aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/12(2015-06-01), 5083-5093 0175-7598 99:12<5083 2015 99 253 https://doi.org/10.1007/s00253-014-6317-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6317-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Loiseau Clémence Equipe Microbiologie de l'Eau, Université de Poitiers, Ecologie & Biologie des Interactions, UMR CNRS 7267, 1 Rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 9, France aut NATIONALLICENCE 700 1- Schlusselhuber Margot Equipe Microbiologie de l'Eau, Université de Poitiers, Ecologie & Biologie des Interactions, UMR CNRS 7267, 1 Rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 9, France aut NATIONALLICENCE 700 1- Bigot Renaud Equipe Microbiologie de l'Eau, Université de Poitiers, Ecologie & Biologie des Interactions, UMR CNRS 7267, 1 Rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 9, France aut NATIONALLICENCE 700 1- Bertaux Joanne Equipe Ecologie Evolution Symbiose, Université de Poitiers, Ecologie & Biologie des Interactions, UMR CNRS 7267, 1 Rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 9, France aut NATIONALLICENCE 700 1- Berjeaud Jean-Marc Equipe Microbiologie de l'Eau, Université de Poitiers, Ecologie & Biologie des Interactions, UMR CNRS 7267, 1 Rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 9, France aut NATIONALLICENCE 700 1- Verdon Julien Equipe Microbiologie de l'Eau, Université de Poitiers, Ecologie & Biologie des Interactions, UMR CNRS 7267, 1 Rue Georges Bonnet, TSA 51106, 86073, Poitiers Cedex 9, France aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/12(2015-06-01), 5083-5093 0175-7598 99:12<5083 2015 99 253