An organic solvent-tolerant phenolic acid decarboxylase from Bacillus licheniformis for the efficient bioconversion of hydroxycinnamic acids to vinyl phenol derivatives
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6313-3 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6313-3 | ||
| 245 | 0 | 3 | |a An organic solvent-tolerant phenolic acid decarboxylase from Bacillus licheniformis for the efficient bioconversion of hydroxycinnamic acids to vinyl phenol derivatives |h [Elektronische Daten] |c [Hongfei Hu, Lulu Li, Shaojun Ding] |
| 520 | 3 | |a A new phenolic acid decarboxylase gene (blpad) from Bacillus licheniformis was cloned and overexpressed in Escherichia coli. The full-length blpad encodes a 166-amino acid polypeptide with a predicted molecular mass and pI of 19,521Da and 5.02, respectively. The recombinant BLPAD displayed maximum activity at 37°C and pH6.0. This enzyme possesses a broad substrate specificity and is able to decarboxylate p-coumaric, ferulic, caffeic, and sinapic acids at the relative ratios of specific activities 100:74.59:34.41:0.29. Kinetic constant K m values toward p-coumaric, ferulic, caffeic, and sinapic acids were 1.64, 1.55, 1.93, and 2.45mM, and V max values were 268.43, 216.80, 119.07, and 0.78Umg−1, respectively. In comparison with other phenolic acid decarboxylases, BLPAD exhibited remarkable organic solvent tolerance and good thermal stability. BLPAD showed excellent catalytic performance in biphasic organic/aqueous systems and efficiently converted p-coumaric and ferulic acids into 4-vinylphenol and 4-vinylguaiacol. At 500mM of p-coumaric and ferulic acids, the recombinant BLPAD produced a total 60.63gl−1 4-vinylphenol and 58.30gl−1 4-vinylguaiacol with the conversion yields 97.02 and 70.96%, respectively. The low yield and product concentration are the crucial drawbacks to the practical bioproduction of vinyl phenol derivatives using phenolic acid decarboxylases. These unusual properties make BLPAD a desirable biocatalyst for commercial use in the bioconversion of hydroxycinnamic acids to vinyl phenol derivatives via enzymatic decarboxylation in a biphasic organic/aqueous reaction system. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a Bacillus licheniformis |2 nationallicence | |
| 690 | 7 | |a Phenolic acid decarboxylase |2 nationallicence | |
| 690 | 7 | |a Hydroxycinnamic acids |2 nationallicence | |
| 690 | 7 | |a Vinyl phenol derivatives |2 nationallicence | |
| 690 | 7 | |a Bioconversion |2 nationallicence | |
| 700 | 1 | |a Hu |D Hongfei |u Department of Biological Engineering, College of Chemical Engineering, Nanjing Forestry University, 210037, Nanjing, Jiangsu, China |4 aut | |
| 700 | 1 | |a Li |D Lulu |u Department of Biological Engineering, College of Chemical Engineering, Nanjing Forestry University, 210037, Nanjing, Jiangsu, China |4 aut | |
| 700 | 1 | |a Ding |D Shaojun |u Department of Biological Engineering, College of Chemical Engineering, Nanjing Forestry University, 210037, Nanjing, Jiangsu, China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/12(2015-06-01), 5071-5081 |x 0175-7598 |q 99:12<5071 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6313-3 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6313-3 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Hu |D Hongfei |u Department of Biological Engineering, College of Chemical Engineering, Nanjing Forestry University, 210037, Nanjing, Jiangsu, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Li |D Lulu |u Department of Biological Engineering, College of Chemical Engineering, Nanjing Forestry University, 210037, Nanjing, Jiangsu, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ding |D Shaojun |u Department of Biological Engineering, College of Chemical Engineering, Nanjing Forestry University, 210037, Nanjing, Jiangsu, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/12(2015-06-01), 5071-5081 |x 0175-7598 |q 99:12<5071 |1 2015 |2 99 |o 253 | ||