caa a22 4500 60550055X CHVBK 20210128100556.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00253-014-6313-3 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6313-3 An organic solvent-tolerant phenolic acid decarboxylase from Bacillus licheniformis for the efficient bioconversion of hydroxycinnamic acids to vinyl phenol derivatives [Elektronische Daten] [Hongfei Hu, Lulu Li, Shaojun Ding] A new phenolic acid decarboxylase gene (blpad) from Bacillus licheniformis was cloned and overexpressed in Escherichia coli. The full-length blpad encodes a 166-amino acid polypeptide with a predicted molecular mass and pI of 19,521Da and 5.02, respectively. The recombinant BLPAD displayed maximum activity at 37°C and pH6.0. This enzyme possesses a broad substrate specificity and is able to decarboxylate p-coumaric, ferulic, caffeic, and sinapic acids at the relative ratios of specific activities 100:74.59:34.41:0.29. Kinetic constant K m values toward p-coumaric, ferulic, caffeic, and sinapic acids were 1.64, 1.55, 1.93, and 2.45mM, and V max values were 268.43, 216.80, 119.07, and 0.78Umg−1, respectively. In comparison with other phenolic acid decarboxylases, BLPAD exhibited remarkable organic solvent tolerance and good thermal stability. BLPAD showed excellent catalytic performance in biphasic organic/aqueous systems and efficiently converted p-coumaric and ferulic acids into 4-vinylphenol and 4-vinylguaiacol. At 500mM of p-coumaric and ferulic acids, the recombinant BLPAD produced a total 60.63gl−1 4-vinylphenol and 58.30gl−1 4-vinylguaiacol with the conversion yields 97.02 and 70.96%, respectively. The low yield and product concentration are the crucial drawbacks to the practical bioproduction of vinyl phenol derivatives using phenolic acid decarboxylases. These unusual properties make BLPAD a desirable biocatalyst for commercial use in the bioconversion of hydroxycinnamic acids to vinyl phenol derivatives via enzymatic decarboxylation in a biphasic organic/aqueous reaction system. Springer-Verlag Berlin Heidelberg, 2014 Bacillus licheniformis nationallicence Phenolic acid decarboxylase nationallicence Hydroxycinnamic acids nationallicence Vinyl phenol derivatives nationallicence Bioconversion nationallicence Hu Hongfei Department of Biological Engineering, College of Chemical Engineering, Nanjing Forestry University, 210037, Nanjing, Jiangsu, China aut Li Lulu Department of Biological Engineering, College of Chemical Engineering, Nanjing Forestry University, 210037, Nanjing, Jiangsu, China aut Ding Shaojun Department of Biological Engineering, College of Chemical Engineering, Nanjing Forestry University, 210037, Nanjing, Jiangsu, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/12(2015-06-01), 5071-5081 0175-7598 99:12<5071 2015 99 253 https://doi.org/10.1007/s00253-014-6313-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6313-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hu Hongfei Department of Biological Engineering, College of Chemical Engineering, Nanjing Forestry University, 210037, Nanjing, Jiangsu, China aut NATIONALLICENCE 700 1- Li Lulu Department of Biological Engineering, College of Chemical Engineering, Nanjing Forestry University, 210037, Nanjing, Jiangsu, China aut NATIONALLICENCE 700 1- Ding Shaojun Department of Biological Engineering, College of Chemical Engineering, Nanjing Forestry University, 210037, Nanjing, Jiangsu, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/12(2015-06-01), 5071-5081 0175-7598 99:12<5071 2015 99 253