caa a22 4500 605500576 CHVBK 20210128100556.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00253-014-6358-3 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6358-3 Effect and behaviour of different substrates in relation to the formation of aerobic granular sludge [Elektronische Daten] [M. Pronk, B. Abbas, S. Al-zuhairy, R. Kraan, R. Kleerebezem, M. van Loosdrecht] When aerobic granular sludge is applied for industrial wastewater treatment, different soluble substrates can be present. For stable granular sludge formation on volatile fatty acids (e.g. acetate), production of storage polymers under anaerobic feeding conditions has been shown to be important. This prevents direct aerobic growth on readily available chemical oxygen demand (COD), which is thought to result in unstable granule formation. Here, we investigate the impact of acetate, methanol, butanol, propanol, propionaldehyde, and valeraldehyde on granular sludge formation at 35°C. Methanogenic archaea, growing on methanol, were present in the aerobic granular sludge system. Methanol was completely converted to methane and carbon dioxide by the methanogenic archaeum Methanomethylovorans uponensis during the 1-h anaerobic feeding period, despite the relative high dissolved oxygen concentration (3.5mg O2 L−1) during the subsequent 2-h aeration period. Propionaldehyde and valeraldehyde were fully disproportionated anaerobically into their corresponding carboxylic acids and alcohols. The organic acids produced were converted to storage polymers, while the alcohols (produced and from influent) were absorbed onto the granular sludge matrix and converted aerobically. Our observations show that easy biodegradable substrates not converted anaerobically into storage polymers could lead to unstable granular sludge formation. However, when the easy biodegradable COD is absorbed in the granules and/or when the substrate is converted by relatively slow growing bacteria in the aerobic period, stable granulation can occur. The Author(s), 2015 Aerobic granular sludge nationallicence Methanol nationallicence Alcohol nationallicence Aldehyde nationallicence Methanogens nationallicence Granule formation nationallicence Industrial wastewater nationallicence Disproportionation nationallicence Feeding strategies nationallicence Pronk M. Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands aut Abbas B. Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands aut Al-zuhairy S. Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands aut Kraan R. Royal HaskoningDHV B.V., P.O Box 1132, 3800 BC, Amersfoort, The Netherlands aut Kleerebezem R. Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands aut van Loosdrecht M. Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/12(2015-06-01), 5257-5268 0175-7598 99:12<5257 2015 99 253 https://doi.org/10.1007/s00253-014-6358-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6358-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pronk M. Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands aut NATIONALLICENCE 700 1- Abbas B. Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands aut NATIONALLICENCE 700 1- Al-zuhairy S. Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands aut NATIONALLICENCE 700 1- Kraan R. Royal HaskoningDHV B.V., P.O Box 1132, 3800 BC, Amersfoort, The Netherlands aut NATIONALLICENCE 700 1- Kleerebezem R. Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands aut NATIONALLICENCE 700 1- van Loosdrecht M. Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/12(2015-06-01), 5257-5268 0175-7598 99:12<5257 2015 99 253