caa a22 4500 605500592 CHVBK 20210128100556.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00253-015-6447-y doi (NATIONALLICENCE)springer-10.1007/s00253-015-6447-y Relationship between acid tolerance and cell membrane in Bifidobacterium , revealed by comparative analysis of acid-resistant derivatives and their parental strains grown in medium with and without Tween 80 [Elektronische Daten] [Xu Yang, Xiaomin Hang, Min Zhang, Xianglong Liu, Hong Yang] The acid tolerance is particularly important for bifidobacteria to function as probiotics because they usually encounter acidic environments in food products and gastrointestinal tract passage. In this study, two acid-resistant derivatives Bifidobacterium longum JDY1017dpH and Bifidobacterium breve BB8dpH, which displayed a stable acid-resistant phenotype, were generated. The relationship between acid tolerance and cell membrane was investigated by comparing the two acid-resistant derivatives and their parental strains grown in medium with and without Tween 80. The fold increase in acid tolerance of the two acid-resistant derivatives relative to their parental strains was much higher when cells were grown in medium with Tween 80 (104 ~ 105-fold) than without Tween 80 (181- and 245-fold). Moreover, when cells were grown in medium with Tween 80, the two acid-resistant derivatives exhibited more C18:1 and cycC19:0, higher mean fatty acid chain length, lower membrane fluidity, and higher expression of cfa gene encoding cyclopropane fatty acid synthase than their parental strains. No significant differences in cell membrane were observed between the two acid-resistant derivatives and their parental strains when cells were grown in medium without Tween 80. The present study revealed that, when cells were grown in medium with Tween 80, the significant fold increase in acid tolerance of the two acid-resistant derivatives was mainly ascribed to the pronounced changes in cell membrane compared with their parental strains. Results presented here could provide a basis for developing new strategies of cell membrane modification to enhance acid tolerance in bifidobacteria. Springer-Verlag Berlin Heidelberg, 2015 Bifidobacteria nationallicence Acid tolerance nationallicence Cell membrane nationallicence Tween 80 nationallicence Yang Xu State Key Laboratory of Microbial Metabolism, and School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd., 200240, Shanghai, People's Republic of China aut Hang Xiaomin Institute of Bio-medicine, Shanghai Jiao Da Onlly Company Limited, 200233, Shanghai, People's Republic of China aut Zhang Min State Key Laboratory of Microbial Metabolism, and School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd., 200240, Shanghai, People's Republic of China aut Liu Xianglong State Key Laboratory of Microbial Metabolism, and School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd., 200240, Shanghai, People's Republic of China aut Yang Hong State Key Laboratory of Microbial Metabolism, and School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd., 200240, Shanghai, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/12(2015-06-01), 5227-5236 0175-7598 99:12<5227 2015 99 253 https://doi.org/10.1007/s00253-015-6447-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6447-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Xu State Key Laboratory of Microbial Metabolism, and School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd., 200240, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Hang Xiaomin Institute of Bio-medicine, Shanghai Jiao Da Onlly Company Limited, 200233, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Min State Key Laboratory of Microbial Metabolism, and School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd., 200240, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Liu Xianglong State Key Laboratory of Microbial Metabolism, and School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd., 200240, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Yang Hong State Key Laboratory of Microbial Metabolism, and School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd., 200240, Shanghai, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/12(2015-06-01), 5227-5236 0175-7598 99:12<5227 2015 99 253