caa a22 4500 605500649 CHVBK 20210128100557.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00253-015-6385-8 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6385-8 Impact of elevated CO2 and N addition on bacteria, fungi, and archaea in a marsh ecosystem with various types of plants [Elektronische Daten] [Seung-Hoon Lee, Seon-Young Kim, Weixing Ding, Hojeong Kang] The individual effects of either elevated CO2 or N deposition on soil microbial communities have been widely studied, but limited information is available regarding the responses of the bacteria, fungi, and archaea communities to both elevated CO2 and N in wetland ecosystems with different types of plants. Using a terminal restriction fragment length polymorphism (T-RFLP) analysis and real-time quantitative PCR (RT-Q-PCR), we compared communities of bacteria, fungi, and archaea in a marsh microcosm with one of seven macrophytes, Typha latifolia, Phragmites japonica, Miscanthus sacchariflorus, Scirpus lacustris, Juncus effusus, Phragmites australis, or Zizania latifolia, after exposing them to eCO2 and/or amended N for 110days. Overall, our results showed that the elevated CO2 and N may affect the bacterial and archaeal communities, while they may not affect the fungal community in terms of both diversity and abundance. The effects of elevated CO2 and N on microbial community vary depending on the plant types, and each microbial community shows different responses to the elevated CO2 and N. In particular, elevated CO2 might force a shift in the archaeal community irrespective of the plant type, and the effect of elevated CO2 was enhanced when combined with the N effect. This study indicates that elevated CO2 and N addition could lead to changes in the community structures of bacteria and archaea. Our results also suggest that the fungal group is less sensitive to external changes, while the bacterial and archaeal groups are more sensitive to them. Finally, the characteristics of the plant type and relevant physicochemical factors induced by the elevated CO2 and N may be important key factors structuring the microbial community's response to environmental change, which implies the need for a more comprehensive approach to understanding the pattern of the wetland response to climate change. Springer-Verlag Berlin Heidelberg, 2015 Elevated CO2 nationallicence N deposition nationallicence Bacteria nationallicence Fungi nationallicence Archaea nationallicence Community composition nationallicence Wetland nationallicence Lee Seung-Hoon School of Civil and Environmental Engineering, Yonsei University, 120-749, Seoul, Korea aut Kim Seon-Young National Institute of Environmental Research, 404-708, Incheon, Korea aut Ding Weixing Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, China aut Kang Hojeong School of Civil and Environmental Engineering, Yonsei University, 120-749, Seoul, Korea aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/12(2015-06-01), 5295-5305 0175-7598 99:12<5295 2015 99 253 https://doi.org/10.1007/s00253-015-6385-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6385-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lee Seung-Hoon School of Civil and Environmental Engineering, Yonsei University, 120-749, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Seon-Young National Institute of Environmental Research, 404-708, Incheon, Korea aut NATIONALLICENCE 700 1- Ding Weixing Institute of Soil Science, Chinese Academy of Sciences, 210008, Nanjing, China aut NATIONALLICENCE 700 1- Kang Hojeong School of Civil and Environmental Engineering, Yonsei University, 120-749, Seoul, Korea aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/12(2015-06-01), 5295-5305 0175-7598 99:12<5295 2015 99 253