caa a22 4500 605500657 CHVBK 20210128100557.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00253-015-6554-9 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6554-9 Effectiveness of direct immobilization of bacterial cells onto material surfaces using the bacterionanofiber protein AtaA [Elektronische Daten] [Katsutoshi Hori, Yuki Ohara, Masahito Ishikawa, Hajime Nakatani] The bacterionanofiber protein AtaA, a member of the trimeric autotransporter adhesin family found in Acinetobacter sp. Tol 5, is responsible for the nonspecific, high adhesiveness and autoagglutination of this strain. Previously, we introduced the ataA gene into the nonadhesive Acinetobacter strain ST-550, which conferred high adhesiveness to this strain, immobilized its cells, and improved indigo productivity due to enhanced tolerance to the toxic substrate. In this study, we again demonstrated the effectiveness of this new microbial immobilization method using AtaA in a number of conditions. AtaA enabled the effective immobilization of growing, resting, and lyophilized cells of a type strain of Acinetobacter, ADP1, which is also intrinsically nonadhesive, onto the surface of several kinds of support ranging from artificial to natural materials and from hydrophobic polyurethane to hydrophilic glass. Immobilization with AtaA enabled exclusive cell growth in the support space and only a few cells existed in the bulk medium. Immobilization of resting cells drastically increased cell concentration, depending on the support material; dry cells of approximately 110g/L could be immobilized onto glass wool. Finally, we demonstrated that ADP1 cells immobilized on polyurethane foam can undergo at least 10 repetitive reactions without inactivation during a 5-h period. Even after drying and storing for 3days, the immobilized cells showed enzymatic activity and an ester hydrolysis reaction was repeated by simply transferring the support with the cells into a fresh reaction buffer. Springer-Verlag Berlin Heidelberg, 2015 Immobilization nationallicence Adhesion nationallicence Bacterionanofiber nationallicence Trimeric autotransporter adhesin nationallicence Hori Katsutoshi Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, 464-8603, Nagoya, Aichi, Japan aut Ohara Yuki Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, 464-8603, Nagoya, Aichi, Japan aut Ishikawa Masahito Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, 464-8603, Nagoya, Aichi, Japan aut Nakatani Hajime Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, 464-8603, Nagoya, Aichi, Japan aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/12(2015-06-01), 5025-5032 0175-7598 99:12<5025 2015 99 253 https://doi.org/10.1007/s00253-015-6554-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6554-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hori Katsutoshi Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, 464-8603, Nagoya, Aichi, Japan aut NATIONALLICENCE 700 1- Ohara Yuki Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, 464-8603, Nagoya, Aichi, Japan aut NATIONALLICENCE 700 1- Ishikawa Masahito Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, 464-8603, Nagoya, Aichi, Japan aut NATIONALLICENCE 700 1- Nakatani Hajime Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, 464-8603, Nagoya, Aichi, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/12(2015-06-01), 5025-5032 0175-7598 99:12<5025 2015 99 253