caa a22 4500 605500673 CHVBK 20210128100557.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00253-015-6634-x doi (NATIONALLICENCE)springer-10.1007/s00253-015-6634-x The gene expression profile of resistant and susceptible Bombyx mori strains reveals cypovirus-associated variations in host gene transcript levels [Elektronische Daten] [Rui Guo, Simei Wang, Renyu Xue, Guangli Cao, Xiaolong Hu, Moli Huang, Yangqi Zhang, Yahong Lu, Liyuan Zhu, Fei Chen, Zi Liang, Sulan Kuang, Chengliang Gong] High-throughput paired-end RNA sequencing (RNA-Seq) was performed to investigate the gene expression profile of a susceptible Bombyx mori strain, Lan5, and a resistant B. mori strain, Ou17, which were both orally infected with B. mori cypovirus (BmCPV) in the midgut. There were 330 and 218 up-regulated genes, while there were 147 and 260 down-regulated genes in the Lan5 and Ou17 strains, respectively. Gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment for differentially expressed genes (DEGs) were carried out. Moreover, gene interaction network (STRING) analyses were performed to analyze the relationships among the shared DEGs. Some of these genes were related and formed a large network, in which the genes for B. mori cuticular protein RR-2 motif 123 (BmCPR123) and the gene for B. mori DNA replication licensing factor Mcm2-like (BmMCM2) were key genes among the common up-regulated DEGs, whereas the gene for B. mori heat shock protein 20.1 (Bmhsp20.1) was the central gene among the shared down-regulated DEGs between Lan5 vs Lan5-CPV and Ou17 vs Ou17-CPV. These findings established a comprehensive database of genes that are differentially expressed in response to BmCPV infection between silkworm strains that differed in resistance to BmCPV and implied that these DEGs might be involved in B. mori immune responses against BmCPV infection. Springer-Verlag Berlin Heidelberg, 2015 Bombyx mori nationallicence Cypovirus nationallicence RNA-Seq nationallicence Gene expression profile nationallicence Guo Rui School of Biology and Basic Medical Science, Soochow University, 215123, Suzhou, China aut Wang Simei School of Biology and Basic Medical Science, Soochow University, 215123, Suzhou, China aut Xue Renyu School of Biology and Basic Medical Science, Soochow University, 215123, Suzhou, China aut Cao Guangli School of Biology and Basic Medical Science, Soochow University, 215123, Suzhou, China aut Hu Xiaolong School of Biology and Basic Medical Science, Soochow University, 215123, Suzhou, China aut Huang Moli School of Biology and Basic Medical Science, Soochow University, 215123, Suzhou, China aut Zhang Yangqi School of Biology and Basic Medical Science, Soochow University, 215123, Suzhou, China aut Lu Yahong School of Biology and Basic Medical Science, Soochow University, 215123, Suzhou, China aut Zhu Liyuan School of Biology and Basic Medical Science, Soochow University, 215123, Suzhou, China aut Chen Fei School of Biology and Basic Medical Science, Soochow University, 215123, Suzhou, China aut Liang Zi School of Biology and Basic Medical Science, Soochow University, 215123, Suzhou, China aut Kuang Sulan School of Biology and Basic Medical Science, Soochow University, 215123, Suzhou, China aut Gong Chengliang School of Biology and Basic Medical Science, Soochow University, 215123, Suzhou, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/12(2015-06-01), 5175-5187 0175-7598 99:12<5175 2015 99 253 https://doi.org/10.1007/s00253-015-6634-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6634-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Guo Rui School of Biology and Basic Medical Science, Soochow University, 215123, Suzhou, China aut NATIONALLICENCE 700 1- Wang Simei School of Biology and Basic Medical Science, Soochow University, 215123, Suzhou, China aut NATIONALLICENCE 700 1- Xue Renyu School of Biology and Basic Medical Science, Soochow University, 215123, Suzhou, China aut NATIONALLICENCE 700 1- Cao Guangli School of Biology and Basic Medical Science, Soochow University, 215123, Suzhou, China aut NATIONALLICENCE 700 1- Hu Xiaolong School of Biology and Basic Medical Science, Soochow University, 215123, Suzhou, China aut NATIONALLICENCE 700 1- Huang Moli School of Biology and Basic Medical Science, Soochow University, 215123, Suzhou, China aut NATIONALLICENCE 700 1- Zhang Yangqi School of Biology and Basic Medical Science, Soochow University, 215123, Suzhou, China aut NATIONALLICENCE 700 1- Lu Yahong School of Biology and Basic Medical Science, Soochow University, 215123, Suzhou, China aut NATIONALLICENCE 700 1- Zhu Liyuan School of Biology and Basic Medical Science, Soochow University, 215123, Suzhou, China aut NATIONALLICENCE 700 1- Chen Fei School of Biology and Basic Medical Science, Soochow University, 215123, Suzhou, China aut NATIONALLICENCE 700 1- Liang Zi School of Biology and Basic Medical Science, Soochow University, 215123, Suzhou, China aut NATIONALLICENCE 700 1- Kuang Sulan School of Biology and Basic Medical Science, Soochow University, 215123, Suzhou, China aut NATIONALLICENCE 700 1- Gong Chengliang School of Biology and Basic Medical Science, Soochow University, 215123, Suzhou, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/12(2015-06-01), 5175-5187 0175-7598 99:12<5175 2015 99 253