caa a22 4500 605500738 CHVBK 20210128100557.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00253-015-6440-5 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6440-5 Enhanced autotrophic astaxanthin production from Haematococcus pluvialis under high temperature via heat stress-driven Haber-Weiss reaction [Elektronische Daten] [Min-Eui Hong, Sung Hwang, Won Chang, Byung Kim, Jeewon Lee, Sang Sim] High temperatures (30-36°C) inhibited astaxanthin accumulation in Haematococcus pluvialis under photoautotrophic conditions. The depression of carotenogenesis was primarily attributed to excess intracellular less reactive oxygen species (LROS; O2 − and H2O2) levels generated under high temperature conditions. Here, we show that the heat stress-driven inefficient astaxanthin production was improved by accelerating the iron-catalyzed Haber-Weiss reaction to convert LROS into more reactive oxygen species (MROS; O2 and OH·), thereby facilitating lipid peroxidation. As a result, during 18days of photoautotrophic induction, the astaxanthin concentration of cells cultured in high temperatures in the presence of iron (450μM) was dramatically increased by 75% (30°C) and 133% (36°C) compared to that of cells exposed to heat stress alone. The heat stress-driven Haber-Weiss reaction will be useful for economically producing astaxanthin by reducing energy cost and enhancing photoautotrophic astaxanthin production, particularly outdoors utilizing natural solar radiation including heat and light for photo-induction of H. pluvialis. Springer-Verlag Berlin Heidelberg, 2015 Haematococcus pluvialis nationallicence Photoautotrophic induction nationallicence Astaxanthin nationallicence High temperature nationallicence Haber-Weiss reaction nationallicence Hong Min-Eui Department of Chemical Engineering, Sungkyunkwan University, 440-746, Suwon, South Korea aut Hwang Sung Mirae Fine Chemical Co. Ltd., 50 Jeyakdanji-ro, Hyangnam-eup, 445-938, Hwasung-si, Gyeonggi-do, South Korea aut Chang Won Research Institute, Korea District Heating Corp., 186 Bundang-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, South Korea aut Kim Byung Department of Chemical Engineering, Sungkyunkwan University, 440-746, Suwon, South Korea aut Lee Jeewon Department of Chemical and Biological Engineering, Korea University, 136-713, Seoul, South Korea aut Sim Sang Department of Chemical and Biological Engineering, Korea University, 136-713, Seoul, South Korea aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/12(2015-06-01), 5203-5215 0175-7598 99:12<5203 2015 99 253 https://doi.org/10.1007/s00253-015-6440-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6440-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hong Min-Eui Department of Chemical Engineering, Sungkyunkwan University, 440-746, Suwon, South Korea aut NATIONALLICENCE 700 1- Hwang Sung Mirae Fine Chemical Co. Ltd., 50 Jeyakdanji-ro, Hyangnam-eup, 445-938, Hwasung-si, Gyeonggi-do, South Korea aut NATIONALLICENCE 700 1- Chang Won Research Institute, Korea District Heating Corp., 186 Bundang-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, South Korea aut NATIONALLICENCE 700 1- Kim Byung Department of Chemical Engineering, Sungkyunkwan University, 440-746, Suwon, South Korea aut NATIONALLICENCE 700 1- Lee Jeewon Department of Chemical and Biological Engineering, Korea University, 136-713, Seoul, South Korea aut NATIONALLICENCE 700 1- Sim Sang Department of Chemical and Biological Engineering, Korea University, 136-713, Seoul, South Korea aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/12(2015-06-01), 5203-5215 0175-7598 99:12<5203 2015 99 253