caa a22 4500 605500754 CHVBK 20210128100557.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00253-014-6326-y doi (NATIONALLICENCE)springer-10.1007/s00253-014-6326-y Size control of in vitro synthesized magnetite crystals by the MamC protein of Magnetococcus marinus strain MC-1 [Elektronische Daten] [C. Valverde-Tercedor, M. Montalbán-López, T. Perez-Gonzalez, M. Sanchez-Quesada, T. Prozorov, E. Pineda-Molina, M. Fernandez-Vivas, A. Rodriguez-Navarro, D. Trubitsyn, Dennis Bazylinski, C. Jimenez-Lopez] Magnetotactic bacteria are a diverse group of prokaryotes that share the unique ability of biomineralizing magnetosomes, which are intracellular, membrane-bounded crystals of either magnetite (Fe3O4) or greigite (Fe3S4). Magnetosome biomineralization is mediated by a number of specific proteins, many of which are localized in the magnetosome membrane, and thus is under strict genetic control. Several studies have partially elucidated the effects of a number of these magnetosome-associated proteins in the control of the size of magnetosome magnetite crystals. However, the effect of MamC, one of the most abundant proteins in the magnetosome membrane, remains unclear. In this present study, magnetite nanoparticles were synthesized inorganically in free-drift experiments at 25°C in the presence of different concentrations of the iron-binding recombinant proteins MamC and MamCnts (MamC without its first transmembrane segment) from the marine, magnetotactic bacterium Magnetococcus marinus strain MC-1 and three commercial proteins [α-lactalbumin (α-Lac), myoglobin (Myo), and lysozyme (Lyz)]. While no effect was observed on the size of magnetite crystals formed in the presence of the commercial proteins, biomimetic synthesis in the presence of MamC and MamCnts at concentrations of 10-60μg/mL resulted in the production of larger and more well-developed magnetite crystals (~30-40nm) compared to those of the control (~20-30nm; magnetite crystals grown protein-free). Our results demonstrate that MamC plays an important role in the control of the size of magnetite crystals and could be utilized in biomimetic synthesis of magnetite nanocrystals. Springer-Verlag Berlin Heidelberg, 2015 Biomimetics nationallicence Biomineralization nationallicence MamC nationallicence Magnetite nanoparticles nationallicence Magnetosomes Magnetococcus marinus strain MC-1 nationallicence Magnetotactic bacteria nationallicence Valverde-Tercedor C. Departamento de Microbiologia, Universidad de Granada, Campus de Fuentenueva s/n, 18071, Granada, Spain aut Montalbán-López M. Departamento de Microbiologia, Universidad de Granada, Campus de Fuentenueva s/n, 18071, Granada, Spain aut Perez-Gonzalez T. Departamento de Microbiologia, Universidad de Granada, Campus de Fuentenueva s/n, 18071, Granada, Spain aut Sanchez-Quesada M. Departamento de Microbiologia, Universidad de Granada, Campus de Fuentenueva s/n, 18071, Granada, Spain aut Prozorov T. US DOE Ames Laboratory, 50011, Ames, IA, USA aut Pineda-Molina E. Laboratorio de Estudios Cristalográficos, IACT, CSIC, Granada, Spain aut Fernandez-Vivas M. Departamento de Microbiologia, Universidad de Granada, Campus de Fuentenueva s/n, 18071, Granada, Spain aut Rodriguez-Navarro A. Departamento de Mineralogía y Petrología, Universidad de Granada, Granada, Spain aut Trubitsyn D. School of Life Sciences, University of Nevada at Las Vegas, Las Vegas, NV, USA aut Bazylinski Dennis School of Life Sciences, University of Nevada at Las Vegas, Las Vegas, NV, USA aut Jimenez-Lopez C. Departamento de Microbiologia, Universidad de Granada, Campus de Fuentenueva s/n, 18071, Granada, Spain aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/12(2015-06-01), 5109-5121 0175-7598 99:12<5109 2015 99 253 https://doi.org/10.1007/s00253-014-6326-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6326-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Valverde-Tercedor C. Departamento de Microbiologia, Universidad de Granada, Campus de Fuentenueva s/n, 18071, Granada, Spain aut NATIONALLICENCE 700 1- Montalbán-López M. Departamento de Microbiologia, Universidad de Granada, Campus de Fuentenueva s/n, 18071, Granada, Spain aut NATIONALLICENCE 700 1- Perez-Gonzalez T. Departamento de Microbiologia, Universidad de Granada, Campus de Fuentenueva s/n, 18071, Granada, Spain aut NATIONALLICENCE 700 1- Sanchez-Quesada M. Departamento de Microbiologia, Universidad de Granada, Campus de Fuentenueva s/n, 18071, Granada, Spain aut NATIONALLICENCE 700 1- Prozorov T. US DOE Ames Laboratory, 50011, Ames, IA, USA aut NATIONALLICENCE 700 1- Pineda-Molina E. Laboratorio de Estudios Cristalográficos, IACT, CSIC, Granada, Spain aut NATIONALLICENCE 700 1- Fernandez-Vivas M. Departamento de Microbiologia, Universidad de Granada, Campus de Fuentenueva s/n, 18071, Granada, Spain aut NATIONALLICENCE 700 1- Rodriguez-Navarro A. Departamento de Mineralogía y Petrología, Universidad de Granada, Granada, Spain aut NATIONALLICENCE 700 1- Trubitsyn D. School of Life Sciences, University of Nevada at Las Vegas, Las Vegas, NV, USA aut NATIONALLICENCE 700 1- Bazylinski Dennis School of Life Sciences, University of Nevada at Las Vegas, Las Vegas, NV, USA aut NATIONALLICENCE 700 1- Jimenez-Lopez C. Departamento de Microbiologia, Universidad de Granada, Campus de Fuentenueva s/n, 18071, Granada, Spain aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/12(2015-06-01), 5109-5121 0175-7598 99:12<5109 2015 99 253