Expanding the threonine aldolase toolbox for the asymmetric synthesis of tertiary α-amino acids
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6803-y |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6803-y | ||
| 245 | 0 | 0 | |a Expanding the threonine aldolase toolbox for the asymmetric synthesis of tertiary α-amino acids |h [Elektronische Daten] |c [Kateryna Fesko, Gernot Strohmeier, Rolf Breinbauer] |
| 520 | 3 | |a The direct biochemical synthesis of tertiary α-amino acids with a wide range of diversity was recently reported using natural threonine aldolases LTA from Aeromonas jandei and DTA from Pseudomonas sp. Here, we describe the identification of five novel threonine aldolases which accept alanine and serine as amino acid donors. The enzymes were found by sequence database analysis using known aldolases as template. All enzymes were overexpressed in Escherichia coli and purified, and their biochemical properties were characterized. The new enantiocomplementary l- and d-threonine aldolases catalyze the asymmetric synthesis of β-hydroxy α-methyl- and α-hydroxymethyl-α-amino acids with good conversion and perfect enantioselectivity at α-carbon of the products (e.e. >99%). The structural basis for the broad donor specificity of these threonine aldolases is analyzed based on crystal structure alignments and amino acid sequences comparison. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Threonine aldolase |2 nationallicence | |
| 690 | 7 | |a Aldol reactions |2 nationallicence | |
| 690 | 7 | |a Tertiary amino acids |2 nationallicence | |
| 690 | 7 | |a Enzyme catalysis |2 nationallicence | |
| 690 | 7 | |a Biocatalysis |2 nationallicence | |
| 700 | 1 | |a Fesko |D Kateryna |u Institute of Organic Chemistry, Graz University of Technology, Stremayrgasse 9, A-8010, Graz, Austria |4 aut | |
| 700 | 1 | |a Strohmeier |D Gernot |u Institute of Organic Chemistry, Graz University of Technology, Stremayrgasse 9, A-8010, Graz, Austria |4 aut | |
| 700 | 1 | |a Breinbauer |D Rolf |u Institute of Organic Chemistry, Graz University of Technology, Stremayrgasse 9, A-8010, Graz, Austria |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/22(2015-11-01), 9651-9661 |x 0175-7598 |q 99:22<9651 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6803-y |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6803-y |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Fesko |D Kateryna |u Institute of Organic Chemistry, Graz University of Technology, Stremayrgasse 9, A-8010, Graz, Austria |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Strohmeier |D Gernot |u Institute of Organic Chemistry, Graz University of Technology, Stremayrgasse 9, A-8010, Graz, Austria |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Breinbauer |D Rolf |u Institute of Organic Chemistry, Graz University of Technology, Stremayrgasse 9, A-8010, Graz, Austria |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/22(2015-11-01), 9651-9661 |x 0175-7598 |q 99:22<9651 |1 2015 |2 99 |o 253 | ||