caa a22 4500 605500827 CHVBK 20210128100557.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00253-015-6824-6 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6824-6 The influence of naphthaleneacetic acid (NAA) and coumarin on flavonoid production by fungus Phellinus sp.: modeling of production kinetic profiles [Elektronische Daten] [Xiao-Kui Ma, Le Li, Eric Peterson, Tingting Ruan, Xiaoyi Duan] For the purpose of improving the fungal production of flavonoids, the influence of naphthaleneacetic acid (NAA) and coumarin on flavonoid production by fungus Phellinus sp. P0988 was investigated by developing the corresponding kinetics of flavonoid production in a 7-L bioreactor. Phellinus sp. was confirmed to form flavonoids in pellets and broth when cultivated in basic medium, and the optimum concentration of NAA and coumarin in medium for flavonoid production were determined to be 0.03 and 0.02g/L, respectively. The developed unstructured mathematical models were in good agreement with the experimental results with respect to flavonoid production kinetic profiles with NAA and coumarin supplementation at optimum levels and revealed significant accuracy in terms of statistical consistency and robustness. Analysis of these kinetic processes indicated that NAA and coumarin supplementations imposed a stronger positive influence on flavonoid production and substrate consumption compared to their effects on cell growth. The separate addition of NAA and coumarin resulted in enhancements in final product accumulation and productivity, achieving final flavonoid concentrations of 3.60 and 2.75g/L, respectively, and glucose consumption showed a significant decrease compared to the non-supplemented control as well. Also, the separate presence of NAA and coumarin respectively decreased maintenance coefficients (M s) from 2.48 in the control to 1.39 and 0.22, representing decreases of 43.9 and 91.1%, respectively. The current study is the first known application of mathematical kinetic models to explore the influence of medium components adding on flavonoid production by fungi. Springer-Verlag Berlin Heidelberg, 2015 Modeling nationallicence Naphthaleneacetic acid (NAA) nationallicence Coumarin nationallicence Filamentous fungi nationallicence Growth kinetics nationallicence Production kinetics nationallicence Ma Xiao-Kui Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, 710062, Xi'an, Shaanxi, China aut Li Le Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, 710062, Xi'an, Shaanxi, China aut Peterson Eric Department of Chemical Engineering, Queen's University, Kingston, Canada aut Ruan Tingting Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, 710062, Xi'an, Shaanxi, China aut Duan Xiaoyi First Affiliated Hospital of Xi'an Jiaotong University, No. 277 West Yanta Road, 710061, Xi'an, Shaanxi, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9417-9426 0175-7598 99:22<9417 2015 99 253 https://doi.org/10.1007/s00253-015-6824-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6824-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ma Xiao-Kui Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, 710062, Xi'an, Shaanxi, China aut NATIONALLICENCE 700 1- Li Le Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, 710062, Xi'an, Shaanxi, China aut NATIONALLICENCE 700 1- Peterson Eric Department of Chemical Engineering, Queen's University, Kingston, Canada aut NATIONALLICENCE 700 1- Ruan Tingting Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, College of Life Sciences, Shaanxi Normal University, 710062, Xi'an, Shaanxi, China aut NATIONALLICENCE 700 1- Duan Xiaoyi First Affiliated Hospital of Xi'an Jiaotong University, No. 277 West Yanta Road, 710061, Xi'an, Shaanxi, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9417-9426 0175-7598 99:22<9417 2015 99 253