caa a22 4500 605500835 CHVBK 20210128100558.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00253-015-6950-1 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6950-1 Metabolic engineering of Bacillus subtilis for terpenoid production [Elektronische Daten] [Zheng Guan, Dan Xue, Ingy Abdallah, Linda Dijkshoorn, Rita Setroikromo, Guiyuan Lv, Wim Quax] Terpenoids are the largest group of small-molecule natural products, with more than 60,000 compounds made from isopentenyl diphosphate (IPP) and its isomer dimethylallyl diphosphate (DMAPP). As the most diverse group of small-molecule natural products, terpenoids play an important role in the pharmaceutical, food, and cosmetic industries. For decades, Escherichia coli (E. coli) and Saccharomyces cerevisiae (S. cerevisiae) were extensively studied to biosynthesize terpenoids, because they are both fully amenable to genetic modifications and have vast molecular resources. On the other hand, our literature survey (20years) revealed that terpenoids are naturally more widespread in Bacillales. In the mid-1990s, an inherent methylerythritol phosphate (MEP) pathway was discovered in Bacillus subtilis (B. subtilis). Since B. subtilis is a generally recognized as safe (GRAS) organism and has long been used for the industrial production of proteins, attempts to biosynthesize terpenoids in this bacterium have aroused much interest in the scientific community. This review discusses metabolic engineering of B. subtilis for terpenoid production, and encountered challenges will be discussed. We will summarize some major advances and outline future directions for exploiting the potential of B. subtilis as a desired "cell factory” to produce terpenoids. The Author(s), 2015 Biosynthesis nationallicence Metabolic engineering nationallicence Terpenoids nationallicence Bacillus subtilis nationallicence Guan Zheng Department of Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, Building 3215, room 917, 9713 AV, Groningen, Netherlands aut Xue Dan Department of Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, Building 3215, room 917, 9713 AV, Groningen, Netherlands aut Abdallah Ingy Department of Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, Building 3215, room 917, 9713 AV, Groningen, Netherlands aut Dijkshoorn Linda Department of Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, Building 3215, room 917, 9713 AV, Groningen, Netherlands aut Setroikromo Rita Department of Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, Building 3215, room 917, 9713 AV, Groningen, Netherlands aut Lv Guiyuan Institute of Materia Medica, Zhejiang Chinese Medical University, 310053, Hangzhou, China aut Quax Wim Department of Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, Building 3215, room 917, 9713 AV, Groningen, Netherlands aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9395-9406 0175-7598 99:22<9395 2015 99 253 https://doi.org/10.1007/s00253-015-6950-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6950-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Guan Zheng Department of Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, Building 3215, room 917, 9713 AV, Groningen, Netherlands aut NATIONALLICENCE 700 1- Xue Dan Department of Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, Building 3215, room 917, 9713 AV, Groningen, Netherlands aut NATIONALLICENCE 700 1- Abdallah Ingy Department of Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, Building 3215, room 917, 9713 AV, Groningen, Netherlands aut NATIONALLICENCE 700 1- Dijkshoorn Linda Department of Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, Building 3215, room 917, 9713 AV, Groningen, Netherlands aut NATIONALLICENCE 700 1- Setroikromo Rita Department of Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, Building 3215, room 917, 9713 AV, Groningen, Netherlands aut NATIONALLICENCE 700 1- Lv Guiyuan Institute of Materia Medica, Zhejiang Chinese Medical University, 310053, Hangzhou, China aut NATIONALLICENCE 700 1- Quax Wim Department of Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, Building 3215, room 917, 9713 AV, Groningen, Netherlands aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9395-9406 0175-7598 99:22<9395 2015 99 253