New primers for detecting and quantifying denitrifying anaerobic methane oxidation archaea in different ecological niches
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| 008 | 210128e20151101xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-015-6893-6 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6893-6 | ||
| 245 | 0 | 0 | |a New primers for detecting and quantifying denitrifying anaerobic methane oxidation archaea in different ecological niches |h [Elektronische Daten] |c [Jing Ding, Zhao-Wei Ding, Liang Fu, Yong-Ze Lu, Shuk Cheng, Raymond Zeng] |
| 520 | 3 | |a The significance of ANME-2d in methane sink in the environment has been overlooked, and there was no any study evaluating the distribution of ANME-2d in the environment. New primers were thus needed to be designed for following research. In this paper, a pair of primers (DP397F and DP569R) was designed to quantify ANME-2d. The specificity and amplification efficiency of this primer pair were acceptable. PCR amplification of another pair of primers (DP142F and DP779R) generated a single, bright targeted band from the enrichment sample, but yielded faint, multiple bands from the environmental samples. Nested PCR was conducted using the primers DP142F/DP779R in the first round and DP142F/DP569R in the second round, which generated a bright targeted band. Further phylogenetic analysis showed that these targeted bands were ANME-2d-related sequences. Real-time PCR showed that the copies of the 16s ribosomal RNA gene of ANME-2d in these samples ranged from 3.72×104 to 2.30×105copiesμg−1 DNA, indicating that the percentage of ANME-2d was greatest in a polluted river sample and least in a rice paddy sample. These results demonstrate that the newly developed real-time PCR primers could sufficiently quantify ANME-2d and that nested PCR with an appropriate combination of the new primers could successfully detect ANME-2d in environmental samples; the latter finding suggests that ANME-2d may spread in environments. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a DAMO |2 nationallicence | |
| 690 | 7 | |a ANME-2d |2 nationallicence | |
| 690 | 7 | |a New primer design |2 nationallicence | |
| 690 | 7 | |a Real-time PCR |2 nationallicence | |
| 690 | 7 | |a Phylogenetic analysis |2 nationallicence | |
| 690 | 7 | |a Environmental samples |2 nationallicence | |
| 700 | 1 | |a Ding |D Jing |u Advanced Laboratory for Environmental Research and Technology, USTC-CityU, 215123, Suzhou, China |4 aut | |
| 700 | 1 | |a Ding |D Zhao-Wei |u CAS Key Laboratory for Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, 230026, Hefei, China |4 aut | |
| 700 | 1 | |a Fu |D Liang |u CAS Key Laboratory for Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, 230026, Hefei, China |4 aut | |
| 700 | 1 | |a Lu |D Yong-Ze |u CAS Key Laboratory for Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, 230026, Hefei, China |4 aut | |
| 700 | 1 | |a Cheng |D Shuk |u Advanced Laboratory for Environmental Research and Technology, USTC-CityU, 215123, Suzhou, China |4 aut | |
| 700 | 1 | |a Zeng |D Raymond |u Advanced Laboratory for Environmental Research and Technology, USTC-CityU, 215123, Suzhou, China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/22(2015-11-01), 9805-9812 |x 0175-7598 |q 99:22<9805 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6893-6 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6893-6 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ding |D Jing |u Advanced Laboratory for Environmental Research and Technology, USTC-CityU, 215123, Suzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ding |D Zhao-Wei |u CAS Key Laboratory for Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, 230026, Hefei, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Fu |D Liang |u CAS Key Laboratory for Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, 230026, Hefei, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Lu |D Yong-Ze |u CAS Key Laboratory for Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, 230026, Hefei, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Cheng |D Shuk |u Advanced Laboratory for Environmental Research and Technology, USTC-CityU, 215123, Suzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zeng |D Raymond |u Advanced Laboratory for Environmental Research and Technology, USTC-CityU, 215123, Suzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/22(2015-11-01), 9805-9812 |x 0175-7598 |q 99:22<9805 |1 2015 |2 99 |o 253 | ||