caa a22 4500 605500878 CHVBK 20210128100558.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00253-015-6851-3 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6851-3 Improved biomass and protein production in solid-state cultures of an Aspergillus sojae strain harboring the Vitreoscilla hemoglobin [Elektronische Daten] [Rodrigo Mora-Lugo, Marvin Madrigal, Vikas Yelemane, Marcelo Fernandez-Lahore] The biotechnological value of Aspergillus sojae ATCC 20235 (A. sojae) for production of pectinases in solid-state fermentation (SSF) has been demonstrated recently. However, a common drawback of fungal solid-state cultures is the poor diffusion of oxygen into the fungi that limits its growth and biological productivity. The bacterial Vitreoscilla hemoglobin (VHb) has favored the metabolism and productivities of various bacterial and yeast strains besides alleviating hypoxic conditions of its native host, but the use of VHb in filamentous fungi still remains poor explored. Based on the known effects of VHb, this study assessed its applicability to improve A. sojae performance in SSF. The VHb gene (vgb) under control of the constitutive Aspergillus nidulants gpdA promoter was introduced into the genome of A. sojae by Agrobacterium-mediated transformation. Successful fungal transformants were identified by fluorescence microscopy and polymerase chain reaction (PCR) analyses. In solid-state cultures, the content of protease, exo-polygalacturonase (exo-PG), and exo-polymethylgalacturonase (exo-PMG) of the transformed fungus (A. sojae vgb+) improved were 26, 60, and 44% higher, respectively, in comparison to its parental strain (A. sojae wt). Similarly, biomass content was also 1.3 times higher in the transformant strain. No significant difference was observed in endo-polygalacturonase (endo-PG) content between both fungal strains, suggesting dissimilar effects of VHb towards different enzymatic productions. Overall, our results show that biomass, protease, and exo-pectinase content of A. sojae in SSF can be improved by transformation with VHb. The Author(s), 2015 Agrobacterium tumefaciens- mediated transformation nationallicence Aspergillus sojae nationallicence Biomass production nationallicence Pectinases and protease nationallicence Solid-state fermentation nationallicence Vitreoscilla hemoglobin nationallicence Mora-Lugo Rodrigo Downstream Bioprocessing Lab, Jacobs University Bremen gGmbH, Bremen, Germany aut Madrigal Marvin Downstream Bioprocessing Lab, Jacobs University Bremen gGmbH, Bremen, Germany aut Yelemane Vikas Downstream Bioprocessing Lab, Jacobs University Bremen gGmbH, Bremen, Germany aut Fernandez-Lahore Marcelo Downstream Bioprocessing Lab, Jacobs University Bremen gGmbH, Bremen, Germany aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9699-9708 0175-7598 99:22<9699 2015 99 253 https://doi.org/10.1007/s00253-015-6851-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6851-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mora-Lugo Rodrigo Downstream Bioprocessing Lab, Jacobs University Bremen gGmbH, Bremen, Germany aut NATIONALLICENCE 700 1- Madrigal Marvin Downstream Bioprocessing Lab, Jacobs University Bremen gGmbH, Bremen, Germany aut NATIONALLICENCE 700 1- Yelemane Vikas Downstream Bioprocessing Lab, Jacobs University Bremen gGmbH, Bremen, Germany aut NATIONALLICENCE 700 1- Fernandez-Lahore Marcelo Downstream Bioprocessing Lab, Jacobs University Bremen gGmbH, Bremen, Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9699-9708 0175-7598 99:22<9699 2015 99 253