caa a22 4500 605500886 CHVBK 20210128100558.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00253-015-6804-x doi (NATIONALLICENCE)springer-10.1007/s00253-015-6804-x The influence of salinity on the abundance, transcriptional activity, and diversity of AOA and AOB in an estuarine sediment: a microcosm study [Elektronische Daten] [Yan Zhang, Lujun Chen, Tianjiao Dai, Jinping Tian, Donghui Wen] Estuarine sediment-seawater microcosms were established to evaluate the influence of salinity on the population, transcriptional activity, and diversity of ammonia-oxidizing archaea (AOA) and bacteria (AOB). AOA was found to show the most abundant and the highest transcriptional activity under moderate salinity; on the other hand, AOB abundance was not sensitive to salinity variation but showed the highest transcriptional activity in the low-salinity microcosms. AOA exhibited more advantages than AOB on growth and ammonia-oxidizing activity under moderate- and high-salinity environments. The highest richness and diversity of active AOA were found under salinity of 15 psu. All the active AOA detected under the salinities studied were clustered into Nitrosopumilus maritimus linage, with the composition shifted from N. maritimus C12 cluster, N. maritimus like 1.1 cluster, N. maritimus SCM1 cluster, and N. maritimus like 1.2 cluster to N. maritimus C12 and N. maritimus A10 clusters when salinity was increased from 5 to 30 psu. Springer-Verlag Berlin Heidelberg, 2015 Ammonia-oxidizing bacteria nationallicence Ammonia-oxidizing archaea nationallicence Estuarine sediment nationallicence Salinity nationallicence Pyrosequecing nationallicence Zhang Yan School of Environment, Tsinghua University, 100084, Beijing, China aut Chen Lujun School of Environment, Tsinghua University, 100084, Beijing, China aut Dai Tianjiao College of Environmental Sciences and Engineering, Peking University, 100871, Beijing, China aut Tian Jinping School of Environment, Tsinghua University, 100084, Beijing, China aut Wen Donghui College of Environmental Sciences and Engineering, Peking University, 100871, Beijing, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9825-9833 0175-7598 99:22<9825 2015 99 253 https://doi.org/10.1007/s00253-015-6804-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6804-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Yan School of Environment, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Chen Lujun School of Environment, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Dai Tianjiao College of Environmental Sciences and Engineering, Peking University, 100871, Beijing, China aut NATIONALLICENCE 700 1- Tian Jinping School of Environment, Tsinghua University, 100084, Beijing, China aut NATIONALLICENCE 700 1- Wen Donghui College of Environmental Sciences and Engineering, Peking University, 100871, Beijing, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9825-9833 0175-7598 99:22<9825 2015 99 253