caa a22 4500 605500894 CHVBK 20210128100558.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00253-015-6841-5 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6841-5 Pilot-scale chitin extraction from shrimp shell waste by deproteination and decalcification with bacterial enrichment cultures [Elektronische Daten] [Mini Bajaj, Andrea Freiberg, Josef Winter, Youmei Xu, Claudia Gallert] Extraction of chitin from mechanically pre-purified shrimp shells can be achieved by successive NaOH/HCl treatment, protease/HCl treatment or by environmentally friendly fermentation with proteolytic/lactic acid bacteria (LAB). For the last mentioned alternative, scale-up of shrimp shell chitin purification was investigated in 0.25L (F1), 10L (F2), and 300L (F3) fermenters using an anaerobic, chitinase-deficient, proteolytic enrichment culture from ground meat for deproteination and a mixed culture of LAB from bio-yoghurt for decalcification. Protein removal in F1, F2, and F3 proceeded in parallel within 40h at an efficiency of 89-91%. Between 85 and 90% of the calcit was removed from the shells by LAB in another 40h in F1, F2, and F3. After deproteination of shrimp shells in F3, spent fermentation liquor was re-used for a next batch of 30-kg shrimp shells in F4 (300L) which eliminated 85.5% protein. The purity of the resulting chitin was comparable in F1, F2, F3, and F4. Viscosities of chitosan, obtained after chitin deacetylation and of chitin, prepared biologically or chemically in the laboratory, were much higher than those of commercially available chitin and chitosan. Springer-Verlag Berlin Heidelberg, 2015 Shrimp shell waste nationallicence Chitin extraction nationallicence Proteolytic enrichment cultures nationallicence Lactobacillus mixed culture nationallicence Pilot-scale fermentation nationallicence Bajaj Mini Institute of Biology for Engineers and Biotechnology of Wastewater Treatment, Karlsruhe Institute of Technology (KIT), Am Fasanengarten, 76131, Karlsruhe, Germany aut Freiberg Andrea Institute of Biology for Engineers and Biotechnology of Wastewater Treatment, Karlsruhe Institute of Technology (KIT), Am Fasanengarten, 76131, Karlsruhe, Germany aut Winter Josef Institute of Biology for Engineers and Biotechnology of Wastewater Treatment, Karlsruhe Institute of Technology (KIT), Am Fasanengarten, 76131, Karlsruhe, Germany aut Xu Youmei Institute of Biology for Engineers and Biotechnology of Wastewater Treatment, Karlsruhe Institute of Technology (KIT), Am Fasanengarten, 76131, Karlsruhe, Germany aut Gallert Claudia Institute of Biology for Engineers and Biotechnology of Wastewater Treatment, Karlsruhe Institute of Technology (KIT), Am Fasanengarten, 76131, Karlsruhe, Germany aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9835-9846 0175-7598 99:22<9835 2015 99 253 https://doi.org/10.1007/s00253-015-6841-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6841-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bajaj Mini Institute of Biology for Engineers and Biotechnology of Wastewater Treatment, Karlsruhe Institute of Technology (KIT), Am Fasanengarten, 76131, Karlsruhe, Germany aut NATIONALLICENCE 700 1- Freiberg Andrea Institute of Biology for Engineers and Biotechnology of Wastewater Treatment, Karlsruhe Institute of Technology (KIT), Am Fasanengarten, 76131, Karlsruhe, Germany aut NATIONALLICENCE 700 1- Winter Josef Institute of Biology for Engineers and Biotechnology of Wastewater Treatment, Karlsruhe Institute of Technology (KIT), Am Fasanengarten, 76131, Karlsruhe, Germany aut NATIONALLICENCE 700 1- Xu Youmei Institute of Biology for Engineers and Biotechnology of Wastewater Treatment, Karlsruhe Institute of Technology (KIT), Am Fasanengarten, 76131, Karlsruhe, Germany aut NATIONALLICENCE 700 1- Gallert Claudia Institute of Biology for Engineers and Biotechnology of Wastewater Treatment, Karlsruhe Institute of Technology (KIT), Am Fasanengarten, 76131, Karlsruhe, Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9835-9846 0175-7598 99:22<9835 2015 99 253