caa a22 4500 605500924 CHVBK 20210128100558.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00253-015-6852-2 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6852-2 Irradiation of Yarrowia lipolytica NRRL YB-567 creating novel strains with enhanced ammonia and oil production on protein and carbohydrate substrates [Elektronische Daten] [Mitch Lindquist, Juan López-Núñez, Marjorie Jones, Elby Cox, Rebecca Pinkelman, Sookie Bang, Bryan Moser, Michael Jackson, Loren Iten, Cletus Kurtzman, Kenneth Bischoff, Siqing Liu, Nasib Qureshi, Kenneth Tasaki, Joseph Rich, Michael Cotta, Badal Saha, Stephen Hughes] Increased interest in sustainable production of renewable diesel and other valuable bioproducts is redoubling efforts to improve economic feasibility of microbial-based oil production. Yarrowia lipolytica is capable of employing a wide variety of substrates to produce oil and valuable co-products. We irradiated Y. lipolytica NRRL YB-567 with UV-C to enhance ammonia (for fertilizer) and lipid (for biodiesel) production on low-cost protein and carbohydrate substrates. The resulting strains were screened for ammonia and oil production using color intensity of indicators on plate assays. Seven mutant strains were selected (based on ammonia assay) and further evaluated for growth rate, ammonia and oil production, soluble protein content, and morphology when grown on liver infusion medium (without sugars), and for growth on various substrates. Strains were identified among these mutants that had a faster doubling time, produced higher maximum ammonia levels (enzyme assay) and more oil (Sudan Black assay), and had higher maximum soluble protein levels (Bradford assay) than wild type. When grown on plates with substrates of interest, all mutant strains showed similar results aerobically to wild-type strain. The mutant strain with the highest oil production and the fastest doubling time was evaluated on coffee waste medium. On this medium, the strain produced 0.12g/L ammonia and 0.20g/L 2-phenylethanol, a valuable fragrance/flavoring, in addition to acylglycerols (oil) containing predominantly C16 and C18 residues. These mutant strains will be investigated further for potential application in commercial biodiesel production. The Author(s), 2015 Yarrowia lipolytica UV-C mutagenesis nationallicence Yarrowia protein utilization nationallicence Acylglycerols from oleaginous yeast nationallicence Yarrowia ammonia production nationallicence Yarrowia carbohydrate substrate utilization nationallicence Lindquist Mitch United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Center for Agricultural Utilization Research (NCAUR), Renewable Product Technology Research Unit, 1815 North University Street, 61604, Peoria, IL, USA aut López-Núñez Juan National Coffee Research Centre - Cenicafe, National Federation of Coffee Growers of Colombia - FNC, Cenicafé Planalto Km 4 vía Antigua Chinchiná, Manizales, Caldas, Colombia aut Jones Marjorie 4160 Department of Chemistry, Illinois State University, 214 Julian Hall, 61790-4160, Normal, IL, USA aut Cox Elby United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Center for Agricultural Utilization Research (NCAUR), Renewable Product Technology Research Unit, 1815 North University Street, 61604, Peoria, IL, USA aut Pinkelman Rebecca South Dakota School of Mines & Technology, Chemical and Biological Engineering, 501 East Saint Joseph Street, 57701-3995, Rapid City, SD, USA aut Bang Sookie South Dakota School of Mines & Technology, Chemical and Biological Engineering, 501 East Saint Joseph Street, 57701-3995, Rapid City, SD, USA aut Moser Bryan USDA, ARS, NCAUR, Bio-oils Research Unit, 1815 North University Street, 61604, Peoria, IL, USA aut Jackson Michael United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Center for Agricultural Utilization Research (NCAUR), Renewable Product Technology Research Unit, 1815 North University Street, 61604, Peoria, IL, USA aut Iten Loren USDA, ARS, NCAUR, Bioenergy Research Unit, 1815 North University Street, 61604, Peoria, IL, USA aut Kurtzman Cletus USDA, ARS, NCAUR, Bacterial Foodborne Pathogens and Mycology Research Unit, 1815 North University Street, 61604, Peoria, IL, USA aut Bischoff Kenneth United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Center for Agricultural Utilization Research (NCAUR), Renewable Product Technology Research Unit, 1815 North University Street, 61604, Peoria, IL, USA aut Liu Siqing United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Center for Agricultural Utilization Research (NCAUR), Renewable Product Technology Research Unit, 1815 North University Street, 61604, Peoria, IL, USA aut Qureshi Nasib USDA, ARS, NCAUR, Bioenergy Research Unit, 1815 North University Street, 61604, Peoria, IL, USA aut Tasaki Kenneth Mitsubishi Chemical, USMC Research & Innovation, 410 Palos Verdes Blvd, 90277, Redondo Beach, CA, USA aut Rich Joseph United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Center for Agricultural Utilization Research (NCAUR), Renewable Product Technology Research Unit, 1815 North University Street, 61604, Peoria, IL, USA aut Cotta Michael USDA, ARS, NCAUR, Bioenergy Research Unit, 1815 North University Street, 61604, Peoria, IL, USA aut Saha Badal USDA, ARS, NCAUR, Bioenergy Research Unit, 1815 North University Street, 61604, Peoria, IL, USA aut Hughes Stephen United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Center for Agricultural Utilization Research (NCAUR), Renewable Product Technology Research Unit, 1815 North University Street, 61604, Peoria, IL, USA aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9723-9743 0175-7598 99:22<9723 2015 99 253 https://doi.org/10.1007/s00253-015-6852-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6852-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lindquist Mitch United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Center for Agricultural Utilization Research (NCAUR), Renewable Product Technology Research Unit, 1815 North University Street, 61604, Peoria, IL, USA aut NATIONALLICENCE 700 1- López-Núñez Juan National Coffee Research Centre - Cenicafe, National Federation of Coffee Growers of Colombia - FNC, Cenicafé Planalto Km 4 vía Antigua Chinchiná, Manizales, Caldas, Colombia aut NATIONALLICENCE 700 1- Jones Marjorie 4160 Department of Chemistry, Illinois State University, 214 Julian Hall, 61790-4160, Normal, IL, USA aut NATIONALLICENCE 700 1- Cox Elby United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Center for Agricultural Utilization Research (NCAUR), Renewable Product Technology Research Unit, 1815 North University Street, 61604, Peoria, IL, USA aut NATIONALLICENCE 700 1- Pinkelman Rebecca South Dakota School of Mines & Technology, Chemical and Biological Engineering, 501 East Saint Joseph Street, 57701-3995, Rapid City, SD, USA aut NATIONALLICENCE 700 1- Bang Sookie South Dakota School of Mines & Technology, Chemical and Biological Engineering, 501 East Saint Joseph Street, 57701-3995, Rapid City, SD, USA aut NATIONALLICENCE 700 1- Moser Bryan USDA, ARS, NCAUR, Bio-oils Research Unit, 1815 North University Street, 61604, Peoria, IL, USA aut NATIONALLICENCE 700 1- Jackson Michael United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Center for Agricultural Utilization Research (NCAUR), Renewable Product Technology Research Unit, 1815 North University Street, 61604, Peoria, IL, USA aut NATIONALLICENCE 700 1- Iten Loren USDA, ARS, NCAUR, Bioenergy Research Unit, 1815 North University Street, 61604, Peoria, IL, USA aut NATIONALLICENCE 700 1- Kurtzman Cletus USDA, ARS, NCAUR, Bacterial Foodborne Pathogens and Mycology Research Unit, 1815 North University Street, 61604, Peoria, IL, USA aut NATIONALLICENCE 700 1- Bischoff Kenneth United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Center for Agricultural Utilization Research (NCAUR), Renewable Product Technology Research Unit, 1815 North University Street, 61604, Peoria, IL, USA aut NATIONALLICENCE 700 1- Liu Siqing United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Center for Agricultural Utilization Research (NCAUR), Renewable Product Technology Research Unit, 1815 North University Street, 61604, Peoria, IL, USA aut NATIONALLICENCE 700 1- Qureshi Nasib USDA, ARS, NCAUR, Bioenergy Research Unit, 1815 North University Street, 61604, Peoria, IL, USA aut NATIONALLICENCE 700 1- Tasaki Kenneth Mitsubishi Chemical, USMC Research & Innovation, 410 Palos Verdes Blvd, 90277, Redondo Beach, CA, USA aut NATIONALLICENCE 700 1- Rich Joseph United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Center for Agricultural Utilization Research (NCAUR), Renewable Product Technology Research Unit, 1815 North University Street, 61604, Peoria, IL, USA aut NATIONALLICENCE 700 1- Cotta Michael USDA, ARS, NCAUR, Bioenergy Research Unit, 1815 North University Street, 61604, Peoria, IL, USA aut NATIONALLICENCE 700 1- Saha Badal USDA, ARS, NCAUR, Bioenergy Research Unit, 1815 North University Street, 61604, Peoria, IL, USA aut NATIONALLICENCE 700 1- Hughes Stephen United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Center for Agricultural Utilization Research (NCAUR), Renewable Product Technology Research Unit, 1815 North University Street, 61604, Peoria, IL, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9723-9743 0175-7598 99:22<9723 2015 99 253