caa a22 4500 605500932 CHVBK 20210128100558.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00253-015-6963-9 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6963-9 Efflux systems in bacteria and their metabolic engineering applications [Elektronische Daten] [Christopher Jones, Néstor Hernández Lozada, Brian Pfleger] The production of valuable chemicals from metabolically engineered microbes can be limited by excretion from the cell. Efflux is often overlooked as a bottleneck in metabolic pathways, despite its impact on alleviating feedback inhibition and product toxicity. In the past, it has been assumed that endogenous efflux pumps and membrane porins can accommodate product efflux rates; however, there are an increasing number of examples wherein overexpressing efflux systems is required to improve metabolite production. In this review, we highlight specific examples from the literature where metabolite export has been studied to identify unknown transporters, increase tolerance to metabolites, and improve the production capabilities of engineered bacteria. The review focuses on the export of a broad spectrum of valuable chemicals including amino acids, sugars, flavins, biofuels, and solvents. The combined set of examples supports the hypothesis that efflux systems can be identified and engineered to confer export capabilities on industrially relevant microbes. Springer-Verlag Berlin Heidelberg, 2015 Efflux nationallicence Toxicity nationallicence Metabolic engineering nationallicence Synthetic biology nationallicence Export nationallicence Transporters nationallicence Jones Christopher Department of Chemical and Biological Engineering, University of Wisconsin—Madison, 53706, Madison, WI, USA aut Hernández Lozada Néstor Department of Chemical and Biological Engineering, University of Wisconsin—Madison, 53706, Madison, WI, USA aut Pfleger Brian Department of Chemical and Biological Engineering, University of Wisconsin—Madison, 53706, Madison, WI, USA aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9381-9393 0175-7598 99:22<9381 2015 99 253 https://doi.org/10.1007/s00253-015-6963-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6963-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jones Christopher Department of Chemical and Biological Engineering, University of Wisconsin—Madison, 53706, Madison, WI, USA aut NATIONALLICENCE 700 1- Hernández Lozada Néstor Department of Chemical and Biological Engineering, University of Wisconsin—Madison, 53706, Madison, WI, USA aut NATIONALLICENCE 700 1- Pfleger Brian Department of Chemical and Biological Engineering, University of Wisconsin—Madison, 53706, Madison, WI, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9381-9393 0175-7598 99:22<9381 2015 99 253