caa a22 4500 605500940 CHVBK 20210128100558.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00253-015-6936-z doi (NATIONALLICENCE)springer-10.1007/s00253-015-6936-z Metabolic responses of Rhodococcus erythropolis PR4 grown on diesel oil and various hydrocarbons [Elektronische Daten] [Krisztián Laczi, Ágnes Kis, Balázs Horváth, Gergely Maróti, Botond Hegedüs, Katalin Perei, Gábor Rákhely] Rhodococcus erythropolis PR4 is able to degrade diesel oil, normal-, iso- and cycloparaffins and aromatic compounds. The complete DNA content of the strain was previously sequenced and numerous oxygenase genes were identified. In order to identify the key elements participating in biodegradation of various hydrocarbons, we performed a comparative whole transcriptome analysis of cells grown on hexadecane, diesel oil and acetate. The transcriptomic data for the most prominent genes were validated by RT-qPCR. The expression of two genes coding for alkane-1-monooxygenase enzymes was highly upregulated in the presence of hydrocarbon substrates. The transcription of eight phylogenetically diverse cytochrome P450 (cyp) genes was upregulated in the presence of diesel oil. The transcript levels of various oxygenase genes were determined in cells grown in an artificial mixture, containing hexadecane, cycloparaffin and aromatic compounds and six cyp genes were induced by this hydrocarbon mixture. Five of them were not upregulated by linear and branched hydrocarbons. The expression of fatty acid synthase I genes was downregulated by hydrocarbon substrates, indicating the utilization of external alkanes for fatty acid synthesis. Moreover, the transcription of genes involved in siderophore synthesis, iron transport and exopolysaccharide biosynthesis was also upregulated, indicating their important role in hydrocarbon metabolism. Based on the results, complex metabolic response profiles were established for cells grown on various hydrocarbons. Our results represent a functional annotation of a rhodococcal genome, provide deeper insight into molecular events in diesel/hydrocarbon utilization and suggest novel target genes for environmental monitoring projects. Springer-Verlag Berlin Heidelberg, 2015 Rhodococcus erythropolis PR4 nationallicence Simultaneous hydrocarbon biodegradation nationallicence Transcriptomics nationallicence Diesel oil decomposition nationallicence Metabolic response nationallicence Oxygenases nationallicence Laczi Krisztián Department of Biotechnology, University of Szeged, Közép fasor 52, H-6726, Szeged, Hungary aut Kis Ágnes Department of Biotechnology, University of Szeged, Közép fasor 52, H-6726, Szeged, Hungary aut Horváth Balázs Institute of Biochemistry, Biological Research Centre Hungarian Academy of Sciences, Temesvári krt 62, H-6726, Szeged, Hungary aut Maróti Gergely Institute of Biochemistry, Biological Research Centre Hungarian Academy of Sciences, Temesvári krt 62, H-6726, Szeged, Hungary aut Hegedüs Botond Department of Biotechnology, University of Szeged, Közép fasor 52, H-6726, Szeged, Hungary aut Perei Katalin Department of Biotechnology, University of Szeged, Közép fasor 52, H-6726, Szeged, Hungary aut Rákhely Gábor Department of Biotechnology, University of Szeged, Közép fasor 52, H-6726, Szeged, Hungary aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9745-9759 0175-7598 99:22<9745 2015 99 253 https://doi.org/10.1007/s00253-015-6936-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6936-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Laczi Krisztián Department of Biotechnology, University of Szeged, Közép fasor 52, H-6726, Szeged, Hungary aut NATIONALLICENCE 700 1- Kis Ágnes Department of Biotechnology, University of Szeged, Közép fasor 52, H-6726, Szeged, Hungary aut NATIONALLICENCE 700 1- Horváth Balázs Institute of Biochemistry, Biological Research Centre Hungarian Academy of Sciences, Temesvári krt 62, H-6726, Szeged, Hungary aut NATIONALLICENCE 700 1- Maróti Gergely Institute of Biochemistry, Biological Research Centre Hungarian Academy of Sciences, Temesvári krt 62, H-6726, Szeged, Hungary aut NATIONALLICENCE 700 1- Hegedüs Botond Department of Biotechnology, University of Szeged, Közép fasor 52, H-6726, Szeged, Hungary aut NATIONALLICENCE 700 1- Perei Katalin Department of Biotechnology, University of Szeged, Közép fasor 52, H-6726, Szeged, Hungary aut NATIONALLICENCE 700 1- Rákhely Gábor Department of Biotechnology, University of Szeged, Közép fasor 52, H-6726, Szeged, Hungary aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9745-9759 0175-7598 99:22<9745 2015 99 253