Unravelling glutathione conjugate catabolism in Saccharomyces cerevisiae
the role of glutathione/dipeptide transporters and vacuolar function in the release of volatile sulfur compounds 3-mercaptohexan-1-ol and 4-mercapto-4-methylpentan-2-one
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6833-5 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6833-5 | ||
| 245 | 0 | 0 | |a Unravelling glutathione conjugate catabolism in Saccharomyces cerevisiae |h [Elektronische Daten] |b the role of glutathione/dipeptide transporters and vacuolar function in the release of volatile sulfur compounds 3-mercaptohexan-1-ol and 4-mercapto-4-methylpentan-2-one |c [Antonio Cordente, Dimitra Capone, Chris Curtin] |
| 520 | 3 | |a Sulfur-containing aroma compounds are key contributors to the flavour of a diverse range of foods and beverages, such as wine. The tropical fruit characters of Sauvignon Blanc wines are attributed to the presence of the aromatic thiols 3-mercaptohexan-1-ol (3-MH), its acetate ester 3-mercaptohexyl acetate (3-MHA), and 4-mercapto-4-methylpentan-2-one (4-MMP). These aromatic thiols are not detectable in grape juice to any significant extent but are released by yeast during alcoholic fermentation. While the processes involved in the release of 3-MH and 4-MMP from their cysteinylated precursors have been studied extensively, degradation pathways for glutathione S-conjugates (GSH-3-MH and GSH-4-MMP) have not. In this study, a candidate gene approach was taken, focusing on genes known to play a role in glutathione and glutathione-S-conjugate turnover in Saccharomyces cerevisiae. Our results confirm the role of Opt1p as the major transporter responsible for uptake of GSH-3-MH and GSH-4-MMP, and identify vacuolar Ecm38p as a key determinant of 3-MH release from GSH-3-MH. ECM38 was unimportant, on the other hand, for release of 4-MMP, and abolition of vacuolar biogenesis caused an increase in the amount of 4-MMP released. The alternative cytosolic glutathione degradation pathway was not involved in release of either thiol from their glutathionylated precursors. Finally, cycling of GSH-3-MH and/or its breakdown intermediates between the cytosol and the vacuole or extracellular space was implicated in modulation of 3-MH formation. Together, these results provide new targets for development of yeast strains that optimize release of these potent volatile sulfur compounds, and further our understanding of the processes involved in glutathione-S-conjugate turnover. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Glutathione- S -conjugate |2 nationallicence | |
| 690 | 7 | |a Volatile thiols |2 nationallicence | |
| 690 | 7 | |a Fermentation |2 nationallicence | |
| 690 | 7 | |a Wine |2 nationallicence | |
| 690 | 7 | |a Yeast |2 nationallicence | |
| 700 | 1 | |a Cordente |D Antonio |u The Australian Wine Research Institute, Glen Osmond, P.O. Box 197, 5064, Adelaide, SA, Australia |4 aut | |
| 700 | 1 | |a Capone |D Dimitra |u The Australian Wine Research Institute, Glen Osmond, P.O. Box 197, 5064, Adelaide, SA, Australia |4 aut | |
| 700 | 1 | |a Curtin |D Chris |u The Australian Wine Research Institute, Glen Osmond, P.O. Box 197, 5064, Adelaide, SA, Australia |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/22(2015-11-01), 9709-9722 |x 0175-7598 |q 99:22<9709 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6833-5 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6833-5 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Cordente |D Antonio |u The Australian Wine Research Institute, Glen Osmond, P.O. Box 197, 5064, Adelaide, SA, Australia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Capone |D Dimitra |u The Australian Wine Research Institute, Glen Osmond, P.O. Box 197, 5064, Adelaide, SA, Australia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Curtin |D Chris |u The Australian Wine Research Institute, Glen Osmond, P.O. Box 197, 5064, Adelaide, SA, Australia |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/22(2015-11-01), 9709-9722 |x 0175-7598 |q 99:22<9709 |1 2015 |2 99 |o 253 | ||