Inactivation of the virulence factors from 2,3-butanediol-producing Klebsiella pneumoniae
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6861-1 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6861-1 | ||
| 245 | 0 | 0 | |a Inactivation of the virulence factors from 2,3-butanediol-producing Klebsiella pneumoniae |h [Elektronische Daten] |c [Duyen Huynh, Ah-Young Kim, In-Hye Seol, Samuel Jung, Min-Cheol Lim, Jeong-A Lee, Mi-Rae Jo, Soo-Jin Choi, Borim Kim, Jinwon Lee, Wooki Kim, Young-Rok Kim] |
| 520 | 3 | |a The microbiological production of 2,3-butanediol (2,3-BDO) has attracted considerable attention as an alternative way to produce high-value chemicals from renewable sources. Among the number of 2,3-BDO-producing microorganisms, Klebsiella pneumoniae has been studied most extensively and is known to produce large quantity of 2,3-BDO from a range of substrates. On the other hand, the pathogenic characteristics of the bacteria have limited its industrial applications. In this study, two major virulence traits, outer core LPS and fimbriae, were removed through homologous recombination from 2,3-BDO-producing K. pneumoniae 2242 to expand its uses to the industrial scale. The K. pneumoniae 2242 ∆wabG mutant strain was found to have an impaired capsule, which significantly reduced its ability to bind to the mucous layer and evade the phagocytic activity of macrophage. The association with the human ileocecal epithelial cell, HCT-8, and the bladder epithelial cell, T-24, was also reduced dramatically in the K. pneumoniae 2242 ∆fimA mutant strain that was devoid of fimbriae. However, the growth rate and production yield for 2,3-BDO were unaffected. The K. pneumoniae strains developed in this study, which are devoid of the major virulence factors, have a high potential for the efficient and sustainable production of 2,3-BDO. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Klebsiella pneumoniae |2 nationallicence | |
| 690 | 7 | |a 2,3-Butanediol |2 nationallicence | |
| 690 | 7 | |a Virulence |2 nationallicence | |
| 690 | 7 | |a Lipopolysaccharides |2 nationallicence | |
| 690 | 7 | |a Capsule |2 nationallicence | |
| 690 | 7 | |a Fimbriae |2 nationallicence | |
| 700 | 1 | |a Huynh |D Duyen |u Graduate School of Biotechnology, Kyung Hee University, 446-701, Yongin, South Korea |4 aut | |
| 700 | 1 | |a Kim |D Ah-Young |u Graduate School of Biotechnology, Kyung Hee University, 446-701, Yongin, South Korea |4 aut | |
| 700 | 1 | |a Seol |D In-Hye |u Graduate School of Biotechnology, Kyung Hee University, 446-701, Yongin, South Korea |4 aut | |
| 700 | 1 | |a Jung |D Samuel |u Graduate School of Biotechnology, Kyung Hee University, 446-701, Yongin, South Korea |4 aut | |
| 700 | 1 | |a Lim |D Min-Cheol |u Graduate School of Biotechnology, Kyung Hee University, 446-701, Yongin, South Korea |4 aut | |
| 700 | 1 | |a Lee |D Jeong-A |u Department of Food Science and Technology, Seoul Women's University, 139-774, Seoul, South Korea |4 aut | |
| 700 | 1 | |a Jo |D Mi-Rae |u Department of Food Science and Technology, Seoul Women's University, 139-774, Seoul, South Korea |4 aut | |
| 700 | 1 | |a Choi |D Soo-Jin |u Department of Food Science and Technology, Seoul Women's University, 139-774, Seoul, South Korea |4 aut | |
| 700 | 1 | |a Kim |D Borim |u Department of Chemical and Biomolecular Engineering, Sogang University, 121-742, Seoul, South Korea |4 aut | |
| 700 | 1 | |a Lee |D Jinwon |u Department of Chemical and Biomolecular Engineering, Sogang University, 121-742, Seoul, South Korea |4 aut | |
| 700 | 1 | |a Kim |D Wooki |u Graduate School of Biotechnology, Kyung Hee University, 446-701, Yongin, South Korea |4 aut | |
| 700 | 1 | |a Kim |D Young-Rok |u Graduate School of Biotechnology, Kyung Hee University, 446-701, Yongin, South Korea |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/22(2015-11-01), 9427-9438 |x 0175-7598 |q 99:22<9427 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6861-1 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6861-1 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Huynh |D Duyen |u Graduate School of Biotechnology, Kyung Hee University, 446-701, Yongin, South Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kim |D Ah-Young |u Graduate School of Biotechnology, Kyung Hee University, 446-701, Yongin, South Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Seol |D In-Hye |u Graduate School of Biotechnology, Kyung Hee University, 446-701, Yongin, South Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Jung |D Samuel |u Graduate School of Biotechnology, Kyung Hee University, 446-701, Yongin, South Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Lim |D Min-Cheol |u Graduate School of Biotechnology, Kyung Hee University, 446-701, Yongin, South Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Lee |D Jeong-A |u Department of Food Science and Technology, Seoul Women's University, 139-774, Seoul, South Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Jo |D Mi-Rae |u Department of Food Science and Technology, Seoul Women's University, 139-774, Seoul, South Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Choi |D Soo-Jin |u Department of Food Science and Technology, Seoul Women's University, 139-774, Seoul, South Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kim |D Borim |u Department of Chemical and Biomolecular Engineering, Sogang University, 121-742, Seoul, South Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Lee |D Jinwon |u Department of Chemical and Biomolecular Engineering, Sogang University, 121-742, Seoul, South Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kim |D Wooki |u Graduate School of Biotechnology, Kyung Hee University, 446-701, Yongin, South Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kim |D Young-Rok |u Graduate School of Biotechnology, Kyung Hee University, 446-701, Yongin, South Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/22(2015-11-01), 9427-9438 |x 0175-7598 |q 99:22<9427 |1 2015 |2 99 |o 253 | ||