caa a22 4500 605500975 CHVBK 20210128100558.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00253-015-6861-1 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6861-1 Inactivation of the virulence factors from 2,3-butanediol-producing Klebsiella pneumoniae [Elektronische Daten] [Duyen Huynh, Ah-Young Kim, In-Hye Seol, Samuel Jung, Min-Cheol Lim, Jeong-A Lee, Mi-Rae Jo, Soo-Jin Choi, Borim Kim, Jinwon Lee, Wooki Kim, Young-Rok Kim] The microbiological production of 2,3-butanediol (2,3-BDO) has attracted considerable attention as an alternative way to produce high-value chemicals from renewable sources. Among the number of 2,3-BDO-producing microorganisms, Klebsiella pneumoniae has been studied most extensively and is known to produce large quantity of 2,3-BDO from a range of substrates. On the other hand, the pathogenic characteristics of the bacteria have limited its industrial applications. In this study, two major virulence traits, outer core LPS and fimbriae, were removed through homologous recombination from 2,3-BDO-producing K. pneumoniae 2242 to expand its uses to the industrial scale. The K. pneumoniae 2242 ∆wabG mutant strain was found to have an impaired capsule, which significantly reduced its ability to bind to the mucous layer and evade the phagocytic activity of macrophage. The association with the human ileocecal epithelial cell, HCT-8, and the bladder epithelial cell, T-24, was also reduced dramatically in the K. pneumoniae 2242 ∆fimA mutant strain that was devoid of fimbriae. However, the growth rate and production yield for 2,3-BDO were unaffected. The K. pneumoniae strains developed in this study, which are devoid of the major virulence factors, have a high potential for the efficient and sustainable production of 2,3-BDO. Springer-Verlag Berlin Heidelberg, 2015 Klebsiella pneumoniae nationallicence 2,3-Butanediol nationallicence Virulence nationallicence Lipopolysaccharides nationallicence Capsule nationallicence Fimbriae nationallicence Huynh Duyen Graduate School of Biotechnology, Kyung Hee University, 446-701, Yongin, South Korea aut Kim Ah-Young Graduate School of Biotechnology, Kyung Hee University, 446-701, Yongin, South Korea aut Seol In-Hye Graduate School of Biotechnology, Kyung Hee University, 446-701, Yongin, South Korea aut Jung Samuel Graduate School of Biotechnology, Kyung Hee University, 446-701, Yongin, South Korea aut Lim Min-Cheol Graduate School of Biotechnology, Kyung Hee University, 446-701, Yongin, South Korea aut Lee Jeong-A Department of Food Science and Technology, Seoul Women's University, 139-774, Seoul, South Korea aut Jo Mi-Rae Department of Food Science and Technology, Seoul Women's University, 139-774, Seoul, South Korea aut Choi Soo-Jin Department of Food Science and Technology, Seoul Women's University, 139-774, Seoul, South Korea aut Kim Borim Department of Chemical and Biomolecular Engineering, Sogang University, 121-742, Seoul, South Korea aut Lee Jinwon Department of Chemical and Biomolecular Engineering, Sogang University, 121-742, Seoul, South Korea aut Kim Wooki Graduate School of Biotechnology, Kyung Hee University, 446-701, Yongin, South Korea aut Kim Young-Rok Graduate School of Biotechnology, Kyung Hee University, 446-701, Yongin, South Korea aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9427-9438 0175-7598 99:22<9427 2015 99 253 https://doi.org/10.1007/s00253-015-6861-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6861-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Huynh Duyen Graduate School of Biotechnology, Kyung Hee University, 446-701, Yongin, South Korea aut NATIONALLICENCE 700 1- Kim Ah-Young Graduate School of Biotechnology, Kyung Hee University, 446-701, Yongin, South Korea aut NATIONALLICENCE 700 1- Seol In-Hye Graduate School of Biotechnology, Kyung Hee University, 446-701, Yongin, South Korea aut NATIONALLICENCE 700 1- Jung Samuel Graduate School of Biotechnology, Kyung Hee University, 446-701, Yongin, South Korea aut NATIONALLICENCE 700 1- Lim Min-Cheol Graduate School of Biotechnology, Kyung Hee University, 446-701, Yongin, South Korea aut NATIONALLICENCE 700 1- Lee Jeong-A Department of Food Science and Technology, Seoul Women's University, 139-774, Seoul, South Korea aut NATIONALLICENCE 700 1- Jo Mi-Rae Department of Food Science and Technology, Seoul Women's University, 139-774, Seoul, South Korea aut NATIONALLICENCE 700 1- Choi Soo-Jin Department of Food Science and Technology, Seoul Women's University, 139-774, Seoul, South Korea aut NATIONALLICENCE 700 1- Kim Borim Department of Chemical and Biomolecular Engineering, Sogang University, 121-742, Seoul, South Korea aut NATIONALLICENCE 700 1- Lee Jinwon Department of Chemical and Biomolecular Engineering, Sogang University, 121-742, Seoul, South Korea aut NATIONALLICENCE 700 1- Kim Wooki Graduate School of Biotechnology, Kyung Hee University, 446-701, Yongin, South Korea aut NATIONALLICENCE 700 1- Kim Young-Rok Graduate School of Biotechnology, Kyung Hee University, 446-701, Yongin, South Korea aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9427-9438 0175-7598 99:22<9427 2015 99 253