caa a22 4500 605500983 CHVBK 20210128100558.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00253-015-6822-8 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6822-8 Degradation of 13C-labeled pyrene in soil-compost mixtures and fertilized soil [Elektronische Daten] [Iris Adam, Anja Miltner, Matthias Kästner] Polycyclic aromatic hydrocarbons (PAH) are toxic pollutants widely distributed in the environment due to natural and anthropogenic processes. In order to mitigate tar oil contaminations with PAH, research on improving bioremediation approaches, which are sometimes inefficient, is needed. However, the knowledge on the fate of PAH-derived carbon and the microbial degraders in particular in compost-supplemented soils is still limited. Here we show the PAH carbon turnover mass balance in microcosms with soil-compost mixtures or in farmyard fertilized soil using [13C6]-pyrene as a model PAH. Complete pyrene degradation of 100mg/kg of soil was observed in all supplemented microcosms within 3 to 5months, and the residual 13C was mainly found as carbon converted to microbial biomass. Long-term fertilization of soil with farmyard manure resulted in pyrene removal efficiency similar to compost addition, although with a much longer lag phase, higher mineralization, and lower carbon incorporation into the biomass. Organic amendments either as long-term manure fertilization or as compost amendment thus play a key role in increasing the PAH-degrading potential of the soil microbial community. Phospholipid fatty acid stable isotope probing (PLFA-SIP) was used to trace the carbon within the microbial population and the amount of biomass formed from pyrene degradation. The results demonstrate that complex microbial degrader consortia rather than the expected single key players are responsible for PAH degradation in organic-amended soil. Springer-Verlag Berlin Heidelberg, 2015 Biodegradation nationallicence Pyrene nationallicence Stable isotope probing nationallicence PLFA nationallicence Carbon mass balance nationallicence Adam Iris Department of Environmental Biotechnology, Helmholtz-Centre for Environmental Research - UFZ, Permoserstr. 15, 04318, Leipzig, Germany aut Miltner Anja Department of Environmental Biotechnology, Helmholtz-Centre for Environmental Research - UFZ, Permoserstr. 15, 04318, Leipzig, Germany aut Kästner Matthias Department of Environmental Biotechnology, Helmholtz-Centre for Environmental Research - UFZ, Permoserstr. 15, 04318, Leipzig, Germany aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9813-9824 0175-7598 99:22<9813 2015 99 253 https://doi.org/10.1007/s00253-015-6822-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6822-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Adam Iris Department of Environmental Biotechnology, Helmholtz-Centre for Environmental Research - UFZ, Permoserstr. 15, 04318, Leipzig, Germany aut NATIONALLICENCE 700 1- Miltner Anja Department of Environmental Biotechnology, Helmholtz-Centre for Environmental Research - UFZ, Permoserstr. 15, 04318, Leipzig, Germany aut NATIONALLICENCE 700 1- Kästner Matthias Department of Environmental Biotechnology, Helmholtz-Centre for Environmental Research - UFZ, Permoserstr. 15, 04318, Leipzig, Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9813-9824 0175-7598 99:22<9813 2015 99 253