caa a22 4500 605501076 CHVBK 20210128100559.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00253-015-6805-9 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6805-9 Genome and secretome analyses provide insights into keratin decomposition by novel proteases from the non-pathogenic fungus Onygena corvina [Elektronische Daten] [Yuhong Huang, Peter Busk, Florian-Alexander Herbst, Lene Lange] Poultry processing plants and slaughterhouses produce huge quantities of feathers and hair/bristle waste annually. These keratinaceous wastes are highly resistant to degradation. Onygena corvina, a non-pathogenic fungus, grows specifically on feathers, hooves, horn, and hair in nature. Hence, the proteases secreted by O. corvina are interesting in view of their potential relevance for industrial decomposition of keratinaceous wastes. We sequenced and assembled the genome of O. corvina and used a method called peptide pattern recognition to identify 73 different proteases. Comparative genome analysis of proteases in keratin-degrading and non-keratin-degrading fungi indicated that 18 putative secreted proteases from four protease families (M36, M35, M43, and S8) may be responsible for keratin decomposition. Twelve of the 18 predicted protease genes could be amplified from O. corvina grown on keratinaceous materials and were transformed into Pichia pastoris. One of the recombinant proteases belonging to the S8 family showed high keratin-degrading activity. Furthermore, 29 different proteases were identified by mass spectrometry in the culture broth of O. corvina grown on feathers and bristle. The culture broth was fractionated by ion exchange chromatography to isolate active fractions with five novel proteases belonging to three protease families (S8, M28, and M3). Enzyme blends composed of three of these five proteases, one from each family, showed high degree of degradation of keratin in vitro. A blend of novel proteases, such as those we discovered, could possibly find a use for degrading keratinaceous wastes and provide proteins, peptides, and amino acids as valuable ingredients for animal feed. The Author(s), 2015 Onygena corvina nationallicence Keratinolytic protease nationallicence Enzyme blends nationallicence Genome nationallicence Peptide pattern recognition nationallicence Secretome nationallicence Huang Yuhong Department of Chemistry and Bioscience, Aalborg University Copenhagen, 2450, Copenhagen, SV, Denmark aut Busk Peter Department of Chemistry and Bioscience, Aalborg University Copenhagen, 2450, Copenhagen, SV, Denmark aut Herbst Florian-Alexander Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, 9220, Aalborg East, Denmark aut Lange Lene Department of Chemistry and Bioscience, Aalborg University Copenhagen, 2450, Copenhagen, SV, Denmark aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9635-9649 0175-7598 99:22<9635 2015 99 253 https://doi.org/10.1007/s00253-015-6805-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6805-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Huang Yuhong Department of Chemistry and Bioscience, Aalborg University Copenhagen, 2450, Copenhagen, SV, Denmark aut NATIONALLICENCE 700 1- Busk Peter Department of Chemistry and Bioscience, Aalborg University Copenhagen, 2450, Copenhagen, SV, Denmark aut NATIONALLICENCE 700 1- Herbst Florian-Alexander Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, 9220, Aalborg East, Denmark aut NATIONALLICENCE 700 1- Lange Lene Department of Chemistry and Bioscience, Aalborg University Copenhagen, 2450, Copenhagen, SV, Denmark aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9635-9649 0175-7598 99:22<9635 2015 99 253