RETRACTED ARTICLE: Enhancement of the catalytic activity of ferulic acid decarboxylase from Enterobacter sp. Px6-4 through random and site-directed mutagenesis
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6717-8 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6717-8 | ||
| 245 | 0 | 0 | |a RETRACTED ARTICLE: Enhancement of the catalytic activity of ferulic acid decarboxylase from Enterobacter sp. Px6-4 through random and site-directed mutagenesis |h [Elektronische Daten] |c [Hyunji Lee, Jiyoung Park, Chaewon Jung, Dongfei Han, Jiyoung Seo, Joong-Hoon Ahn, Youhoon Chong, Hor-Gil Hur] |
| 520 | 3 | |a The enzyme ferulic acid decarboxylase (FADase) from Enterobacter sp. Px6-4 catalyzes the decarboxylation reaction of lignin monomers and phenolic compounds such as p-coumaric acid, caffeic acid, and ferulic acid into their corresponding 4-vinyl derivatives, that is, 4-vinylphenol, 4-vinylcatechol, and 4-vinylguaiacol, respectively. Among various ferulic acid decarboxylase enzymes, we chose the FADase from Enterobacter sp. Px6-4, whose crystal structure is known, and produced mutants to enhance its catalytic activity by random and site-directed mutagenesis. After three rounds of sequential mutations, FADase(F95L/D112N/V151I) showed approximately 34-fold higher catalytic activity than wild-type for the production of 4-vinylguaiacol from ferulic acid. Docking analyses suggested that the increased activity of FADase(F95L/D112N/V151I) could be due to formation of compact active site compared with that of the wild-type FADase. Considering the amount of phenolic compounds such as lignin monomers in the biomass components, successfully bioengineered FADase(F95L/D112N/V151I) from Enterobacter sp. Px6-4 could provide an ecofriendly biocatalytic tool for producing diverse styrene derivatives from biomass. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Ferulic acid decarboxylase |2 nationallicence | |
| 690 | 7 | |a Directed mutagenesis |2 nationallicence | |
| 690 | 7 | |a Ferulic acid |2 nationallicence | |
| 690 | 7 | |a 4-Vinylguaiacol |2 nationallicence | |
| 690 | 7 | |a Biotransformation |2 nationallicence | |
| 700 | 1 | |a Lee |D Hyunji |u School of Environmental Science and Engineering, Gwangju Institute of Science and Technology, 500-712, Gwangju, South Korea |4 aut | |
| 700 | 1 | |a Park |D Jiyoung |u School of Environmental Science and Engineering, Gwangju Institute of Science and Technology, 500-712, Gwangju, South Korea |4 aut | |
| 700 | 1 | |a Jung |D Chaewon |u School of Environmental Science and Engineering, Gwangju Institute of Science and Technology, 500-712, Gwangju, South Korea |4 aut | |
| 700 | 1 | |a Han |D Dongfei |u Max-Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Street 10, D35043, Marburg, Germany |4 aut | |
| 700 | 1 | |a Seo |D Jiyoung |u Radiation Research Division of Industry and Environment, Korea Atomic Energy Research Institute, 1266, 580-185, Jeongup, South Korea |4 aut | |
| 700 | 1 | |a Ahn |D Joong-Hoon |u Department of Bioscience and Biotechnology, Konkuk University, 143-701, Seoul, South Korea |4 aut | |
| 700 | 1 | |a Chong |D Youhoon |u Department of Bioscience and Biotechnology, Konkuk University, 143-701, Seoul, South Korea |4 aut | |
| 700 | 1 | |a Hur |D Hor-Gil |u School of Environmental Science and Engineering, Gwangju Institute of Science and Technology, 500-712, Gwangju, South Korea |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/22(2015-11-01), 9473-9481 |x 0175-7598 |q 99:22<9473 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6717-8 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6717-8 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Lee |D Hyunji |u School of Environmental Science and Engineering, Gwangju Institute of Science and Technology, 500-712, Gwangju, South Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Park |D Jiyoung |u School of Environmental Science and Engineering, Gwangju Institute of Science and Technology, 500-712, Gwangju, South Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Jung |D Chaewon |u School of Environmental Science and Engineering, Gwangju Institute of Science and Technology, 500-712, Gwangju, South Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Han |D Dongfei |u Max-Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Street 10, D35043, Marburg, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Seo |D Jiyoung |u Radiation Research Division of Industry and Environment, Korea Atomic Energy Research Institute, 1266, 580-185, Jeongup, South Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ahn |D Joong-Hoon |u Department of Bioscience and Biotechnology, Konkuk University, 143-701, Seoul, South Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Chong |D Youhoon |u Department of Bioscience and Biotechnology, Konkuk University, 143-701, Seoul, South Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Hur |D Hor-Gil |u School of Environmental Science and Engineering, Gwangju Institute of Science and Technology, 500-712, Gwangju, South Korea |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/22(2015-11-01), 9473-9481 |x 0175-7598 |q 99:22<9473 |1 2015 |2 99 |o 253 | ||