caa a22 4500 605501084 CHVBK 20210128100559.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00253-015-6717-8 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6717-8 RETRACTED ARTICLE: Enhancement of the catalytic activity of ferulic acid decarboxylase from Enterobacter sp. Px6-4 through random and site-directed mutagenesis [Elektronische Daten] [Hyunji Lee, Jiyoung Park, Chaewon Jung, Dongfei Han, Jiyoung Seo, Joong-Hoon Ahn, Youhoon Chong, Hor-Gil Hur] The enzyme ferulic acid decarboxylase (FADase) from Enterobacter sp. Px6-4 catalyzes the decarboxylation reaction of lignin monomers and phenolic compounds such as p-coumaric acid, caffeic acid, and ferulic acid into their corresponding 4-vinyl derivatives, that is, 4-vinylphenol, 4-vinylcatechol, and 4-vinylguaiacol, respectively. Among various ferulic acid decarboxylase enzymes, we chose the FADase from Enterobacter sp. Px6-4, whose crystal structure is known, and produced mutants to enhance its catalytic activity by random and site-directed mutagenesis. After three rounds of sequential mutations, FADase(F95L/D112N/V151I) showed approximately 34-fold higher catalytic activity than wild-type for the production of 4-vinylguaiacol from ferulic acid. Docking analyses suggested that the increased activity of FADase(F95L/D112N/V151I) could be due to formation of compact active site compared with that of the wild-type FADase. Considering the amount of phenolic compounds such as lignin monomers in the biomass components, successfully bioengineered FADase(F95L/D112N/V151I) from Enterobacter sp. Px6-4 could provide an ecofriendly biocatalytic tool for producing diverse styrene derivatives from biomass. Springer-Verlag Berlin Heidelberg, 2015 Ferulic acid decarboxylase nationallicence Directed mutagenesis nationallicence Ferulic acid nationallicence 4-Vinylguaiacol nationallicence Biotransformation nationallicence Lee Hyunji School of Environmental Science and Engineering, Gwangju Institute of Science and Technology, 500-712, Gwangju, South Korea aut Park Jiyoung School of Environmental Science and Engineering, Gwangju Institute of Science and Technology, 500-712, Gwangju, South Korea aut Jung Chaewon School of Environmental Science and Engineering, Gwangju Institute of Science and Technology, 500-712, Gwangju, South Korea aut Han Dongfei Max-Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Street 10, D35043, Marburg, Germany aut Seo Jiyoung Radiation Research Division of Industry and Environment, Korea Atomic Energy Research Institute, 1266, 580-185, Jeongup, South Korea aut Ahn Joong-Hoon Department of Bioscience and Biotechnology, Konkuk University, 143-701, Seoul, South Korea aut Chong Youhoon Department of Bioscience and Biotechnology, Konkuk University, 143-701, Seoul, South Korea aut Hur Hor-Gil School of Environmental Science and Engineering, Gwangju Institute of Science and Technology, 500-712, Gwangju, South Korea aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9473-9481 0175-7598 99:22<9473 2015 99 253 https://doi.org/10.1007/s00253-015-6717-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6717-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lee Hyunji School of Environmental Science and Engineering, Gwangju Institute of Science and Technology, 500-712, Gwangju, South Korea aut NATIONALLICENCE 700 1- Park Jiyoung School of Environmental Science and Engineering, Gwangju Institute of Science and Technology, 500-712, Gwangju, South Korea aut NATIONALLICENCE 700 1- Jung Chaewon School of Environmental Science and Engineering, Gwangju Institute of Science and Technology, 500-712, Gwangju, South Korea aut NATIONALLICENCE 700 1- Han Dongfei Max-Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Street 10, D35043, Marburg, Germany aut NATIONALLICENCE 700 1- Seo Jiyoung Radiation Research Division of Industry and Environment, Korea Atomic Energy Research Institute, 1266, 580-185, Jeongup, South Korea aut NATIONALLICENCE 700 1- Ahn Joong-Hoon Department of Bioscience and Biotechnology, Konkuk University, 143-701, Seoul, South Korea aut NATIONALLICENCE 700 1- Chong Youhoon Department of Bioscience and Biotechnology, Konkuk University, 143-701, Seoul, South Korea aut NATIONALLICENCE 700 1- Hur Hor-Gil School of Environmental Science and Engineering, Gwangju Institute of Science and Technology, 500-712, Gwangju, South Korea aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9473-9481 0175-7598 99:22<9473 2015 99 253