Molecular weight-dependent degradation of d -lactate-containing polyesters by polyhydroxyalkanoate depolymerases from Variovorax sp. C34 and Alcaligenes faecalis T1
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6756-1 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6756-1 | ||
| 245 | 0 | 0 | |a Molecular weight-dependent degradation of d -lactate-containing polyesters by polyhydroxyalkanoate depolymerases from Variovorax sp. C34 and Alcaligenes faecalis T1 |h [Elektronische Daten] |c [Jian Sun, Ken'ichiro Matsumoto, Yuta Tabata, Ryosuke Kadoya, Toshihiko Ooi, Hideki Abe, Seiichi Taguchi] |
| 520 | 3 | |a Polyhydroxyalkanoate depolymerase derived from Variovorax sp. C34 (PhaZVs) was identified as the first enzyme that is capable of degrading isotactic P[67mol% (R)-lactate(LA)-co-(R)-3-hydroxybutyrate(3HB)] [P(d-LA-co-d-3HB)]. This study aimed at analyzing the monomer sequence specificity of PhaZVs for hydrolyzing P(LA-co-3HB) in comparison with a P(3HB) depolymerase from Alcaligenes faecalis T1 (PhaZAf) that did not degrade the same copolymer. Degradation of P(LA-co-3HB) by action of PhaZVs generated dimers, 3HB-3HB, 3HB-LA, LA-3HB, and LA-LA, and the monomers, suggesting that PhaZVs cleaved the linkages between LA and 3HB units and between LA units. To provide a direct evidence for the hydrolysis of these sequences, the synthetic methyl trimers, 3HB-3HB-3HB, LA-LA-3HB, LA-3HB-LA, and 3HB-LA-LA, were treated with the PhaZs. Unexpectedly, not only PhaZVs but also PhaZAf hydrolyzed all of these substrates, namely PhaZAf also cleaved LA-LA linkage. Considering the fact that both PhaZs did not degrade P[(R)-LA] (PDLA) homopolymer, the cleavage capability of LA-LA linkage by PhaZs was supposed to depend on the length of the LA-clustering region in the polymer chain. To test this hypothesis, PDLA oligomers (6 to 40mer) were subjected to the PhaZ assay, revealing that there was an inverse relationship between molecular weight of the substrates and their hydrolysis efficiency. Moreover, PhaZVs exhibited the degrading activity toward significantly longer PDLA oligomers compared to PhaZAf. Therefore, the cleaving capability of PhaZs used here toward the d-LA-based polymers containing the LA-clustering region was strongly associated with the substrate length, rather than the monomer sequence specificity of the enzyme. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Biobased polyester |2 nationallicence | |
| 690 | 7 | |a Polylactic acid |2 nationallicence | |
| 690 | 7 | |a Biodegradation |2 nationallicence | |
| 690 | 7 | |a Biodegradable material |2 nationallicence | |
| 690 | 7 | |a Size-dependent hydrolysis |2 nationallicence | |
| 690 | 7 | |a Monomer sequence specificity |2 nationallicence | |
| 700 | 1 | |a Sun |D Jian |u Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13-W8, Kita-ku, 060-8628, Sapporo, Japan |4 aut | |
| 700 | 1 | |a Matsumoto |D Ken'ichiro |u Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13-W8, Kita-ku, 060-8628, Sapporo, Japan |4 aut | |
| 700 | 1 | |a Tabata |D Yuta |u Department of Innovative and Engineering Materials, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, 226-8502, Yokohama, Kanagawa, Japan |4 aut | |
| 700 | 1 | |a Kadoya |D Ryosuke |u Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13-W8, Kita-ku, 060-8628, Sapporo, Japan |4 aut | |
| 700 | 1 | |a Ooi |D Toshihiko |u Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13-W8, Kita-ku, 060-8628, Sapporo, Japan |4 aut | |
| 700 | 1 | |a Abe |D Hideki |u Department of Innovative and Engineering Materials, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, 226-8502, Yokohama, Kanagawa, Japan |4 aut | |
| 700 | 1 | |a Taguchi |D Seiichi |u Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13-W8, Kita-ku, 060-8628, Sapporo, Japan |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/22(2015-11-01), 9555-9563 |x 0175-7598 |q 99:22<9555 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6756-1 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6756-1 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sun |D Jian |u Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13-W8, Kita-ku, 060-8628, Sapporo, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Matsumoto |D Ken'ichiro |u Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13-W8, Kita-ku, 060-8628, Sapporo, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Tabata |D Yuta |u Department of Innovative and Engineering Materials, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, 226-8502, Yokohama, Kanagawa, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kadoya |D Ryosuke |u Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13-W8, Kita-ku, 060-8628, Sapporo, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ooi |D Toshihiko |u Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13-W8, Kita-ku, 060-8628, Sapporo, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Abe |D Hideki |u Department of Innovative and Engineering Materials, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, 226-8502, Yokohama, Kanagawa, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Taguchi |D Seiichi |u Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13-W8, Kita-ku, 060-8628, Sapporo, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/22(2015-11-01), 9555-9563 |x 0175-7598 |q 99:22<9555 |1 2015 |2 99 |o 253 | ||