caa a22 4500 605501106 CHVBK 20210128100559.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00253-015-6802-z doi (NATIONALLICENCE)springer-10.1007/s00253-015-6802-z Selective production of rubusoside from stevioside by using the sophorose activity of β-glucosidase from Streptomyces sp. GXT6 [Elektronische Daten] [Zilong Wang, Jinpei Wang, Minhua Jiang, Yutuo Wei, Hao Pang, Hang Wei, Ribo Huang, Liqin Du] In order to produce rubusoside, enzymes with preferential specificity for the saccharide sophorose were tested for ability to produce rubusoside from stevioside. We identified BGL1, a β-glucosidase from Streptomyces sp. GXT6, as an enzyme for rubusoside production. Out of several saccharide substrates, BGL1 showed the most affinity to sophorose. This enzyme only hydrolyzes the glucose moiety of the sophoroside at C-13 in stevioside. Production of rubusoside was determined by 1H and 13C nuclear magnetic resonance (NMR). Thus, rubusoside was produced from stevioside and the stevioside conversion rate was 98.2%. The production yield of rubusoside was 78.8% in 6h. Springer-Verlag Berlin Heidelberg, 2015 Streptomyces sp nationallicence β-Glucosidase nationallicence Rubusoside nationallicence Stevioside nationallicence Sophorose nationallicence Rubus suavissimus nationallicence Wang Zilong State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, 530005, Nanning, Guangxi, China aut Wang Jinpei State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, 530005, Nanning, Guangxi, China aut Jiang Minhua State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, 530005, Nanning, Guangxi, China aut Wei Yutuo State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, 530005, Nanning, Guangxi, China aut Pang Hao National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, 98 Daling Road, 530007, Nanning, Guangxi, China aut Wei Hang State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, 530005, Nanning, Guangxi, China aut Huang Ribo State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, 530005, Nanning, Guangxi, China aut Du Liqin State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, 530005, Nanning, Guangxi, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9663-9674 0175-7598 99:22<9663 2015 99 253 https://doi.org/10.1007/s00253-015-6802-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6802-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Zilong State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, 530005, Nanning, Guangxi, China aut NATIONALLICENCE 700 1- Wang Jinpei State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, 530005, Nanning, Guangxi, China aut NATIONALLICENCE 700 1- Jiang Minhua State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, 530005, Nanning, Guangxi, China aut NATIONALLICENCE 700 1- Wei Yutuo State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, 530005, Nanning, Guangxi, China aut NATIONALLICENCE 700 1- Pang Hao National Engineering Research Center for Non-Food Biorefinery, Guangxi Academy of Sciences, 98 Daling Road, 530007, Nanning, Guangxi, China aut NATIONALLICENCE 700 1- Wei Hang State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, 530005, Nanning, Guangxi, China aut NATIONALLICENCE 700 1- Huang Ribo State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, 530005, Nanning, Guangxi, China aut NATIONALLICENCE 700 1- Du Liqin State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, 530005, Nanning, Guangxi, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9663-9674 0175-7598 99:22<9663 2015 99 253