caa a22 4500 605501130 CHVBK 20210128100559.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00253-015-6853-1 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6853-1 Microencapsulation of new probiotic formulations for gastrointestinal delivery: in vitro study to assess viability and biological properties [Elektronische Daten] [G. D'Orazio, P. Di Gennaro, M. Boccarusso, I. Presti, G. Bizzaro, S. Giardina, A. Michelotti, M. Labra, B. La Ferla] The paper describes the preparation of new probiotic formulations based on chitosan-coated alginate microcapsules containing three different probiotic strains, Lactobacillus plantarum PBS067, Lactobacillus rhamnosus PBS070, and Bifidobacterium animalis subsp. lactis PBS075 taken individually and as a mixture of them. The effects of microencapsulation on the viability of the strains in conditions simulating the gastrointestinal tract and under industrial processes conditions were studied. In addition, an evaluation of their probiotic properties was also investigated by in vitro tests on the human intestinal cell line HT-29 to explore the effect of microencapsulation on health beneficial effect of the considered strains. Non-encapsulated cells were completely destroyed when exposed to simulated gastric juice and other stress conditions, while encapsulated cells exhibited a significantly higher resistance to artificial intestinal juice and heat and osmotic treatment. Moreover, in this study, the effect of the various microencapsulated probiotic strain formulations was compared with analogous formulations also containing the β-glucan Pleuran. The microencapsulation effectively protected the selected bacteria, as single strain and as a mixture of the three strains in both the formulations with and without Pleuran, from simulating gastrointestinal tract and industrial process conditions in delivering the viable cells without any significant adverse effect on their functionalities. The comparative study of the immunomodulatory properties of each single strain and the mixture of the three strains revealed a synergistic effect of the probiotic mixture, but no appreciable difference between the two kinds of formulations could be detected, as the effect of Pleuran is covered by the higher potential of the probiotic strains. Springer-Verlag Berlin Heidelberg, 2015 Probiotics nationallicence Intestinal microbiota nationallicence Microencapsulated bacteria nationallicence Microencapsulation polymers nationallicence D'Orazio G. Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126, Milan, Italy aut Di Gennaro P. Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126, Milan, Italy aut Boccarusso M. Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126, Milan, Italy aut Presti I. Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126, Milan, Italy aut Bizzaro G. Farcoderm Srl, San Martino Siccomario, Pavia, Italy aut Giardina S. Farcoderm Srl, San Martino Siccomario, Pavia, Italy aut Michelotti A. Farcoderm Srl, San Martino Siccomario, Pavia, Italy aut Labra M. Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126, Milan, Italy aut La Ferla B. Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126, Milan, Italy aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9779-9789 0175-7598 99:22<9779 2015 99 253 https://doi.org/10.1007/s00253-015-6853-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6853-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- D'Orazio G. Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126, Milan, Italy aut NATIONALLICENCE 700 1- Di Gennaro P. Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126, Milan, Italy aut NATIONALLICENCE 700 1- Boccarusso M. Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126, Milan, Italy aut NATIONALLICENCE 700 1- Presti I. Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126, Milan, Italy aut NATIONALLICENCE 700 1- Bizzaro G. Farcoderm Srl, San Martino Siccomario, Pavia, Italy aut NATIONALLICENCE 700 1- Giardina S. Farcoderm Srl, San Martino Siccomario, Pavia, Italy aut NATIONALLICENCE 700 1- Michelotti A. Farcoderm Srl, San Martino Siccomario, Pavia, Italy aut NATIONALLICENCE 700 1- Labra M. Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126, Milan, Italy aut NATIONALLICENCE 700 1- La Ferla B. Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126, Milan, Italy aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9779-9789 0175-7598 99:22<9779 2015 99 253