caa a22 4500 605501173 CHVBK 20210128100559.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00253-015-6947-9 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6947-9 Advances and needs for endotoxin-free production strains [Elektronische Daten] [Seiichi Taguchi, Toshihiko Ooi, Kouhei Mizuno, Hiromi Matsusaki] The choice of an appropriate microbial host cell and suitable production conditions is crucial for the downstream processing of pharmaceutical- and food-grade products. Although Escherichia coli serves as a highly valuable leading platform for the production of value-added products, like most Gram-negative bacteria, this bacterium contains a potent immunostimulatory lipopolysaccharide (LPS), referred to as an endotoxin. In contrast, Gram-positive bacteria, notably Bacillus, lactic acid bacteria (LAB), Corynebacterium, and yeasts have been extensively used as generally recognized as safe (GRAS) endotoxin-free platforms for the production of a variety of products. This review summarizes the currently available knowledge on the utilization of these representative Gram-positive bacteria for the production of eco- and bio-friendly products, particularly natural polyesters, polyhydroxyalkanoates, bacteriocins, and membrane proteins. The successful case studies presented here serve to inspire the use of these microorganisms as a main-player or by-player depending on their individual properties for the industrial production of these desirable targets. Springer-Verlag Berlin Heidelberg, 2015 Food-grade Gram-positive bacteria nationallicence Lipopolysaccharide nationallicence Polyhydroxyalkanoates nationallicence Bacteriocins nationallicence Membrane proteins nationallicence Yeasts nationallicence Taguchi Seiichi Division of Applied Chemistry, Graduate School of Engineering, Hokkaido University, N13-W8, Kita-ku, 060-8628, Sapporo, Japan aut Ooi Toshihiko Division of Applied Chemistry, Graduate School of Engineering, Hokkaido University, N13-W8, Kita-ku, 060-8628, Sapporo, Japan aut Mizuno Kouhei Department of Materials Science and Chemical Engineering, Kitakyushu National College of Technology, 5-20-1 Shii, Kokuraminami-ku, 802-0985, Kitakyushu, Japan aut Matsusaki Hiromi Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto, 3-1-100 Tsukide, Higashi-ku, 862-8502, Kumamoto, Japan aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9349-9360 0175-7598 99:22<9349 2015 99 253 https://doi.org/10.1007/s00253-015-6947-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6947-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Taguchi Seiichi Division of Applied Chemistry, Graduate School of Engineering, Hokkaido University, N13-W8, Kita-ku, 060-8628, Sapporo, Japan aut NATIONALLICENCE 700 1- Ooi Toshihiko Division of Applied Chemistry, Graduate School of Engineering, Hokkaido University, N13-W8, Kita-ku, 060-8628, Sapporo, Japan aut NATIONALLICENCE 700 1- Mizuno Kouhei Department of Materials Science and Chemical Engineering, Kitakyushu National College of Technology, 5-20-1 Shii, Kokuraminami-ku, 802-0985, Kitakyushu, Japan aut NATIONALLICENCE 700 1- Matsusaki Hiromi Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto, 3-1-100 Tsukide, Higashi-ku, 862-8502, Kumamoto, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9349-9360 0175-7598 99:22<9349 2015 99 253