caa a22 4500 605501181 CHVBK 20210128100559.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00253-015-6878-5 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6878-5 A novel aldose-aldose oxidoreductase for co-production of D-xylonate and xylitol from D-xylose with Saccharomyces cerevisiae [Elektronische Daten] [Marilyn Wiebe, Yvonne Nygård, Merja Oja, Martina Andberg, Laura Ruohonen, Anu Koivula, Merja Penttilä, Mervi Toivari] An open reading frame CC1225 from the Caulobacter crescentus CB15 genome sequence belongs to the Gfo/Idh/MocA protein family and has 47% amino acid sequence identity with the glucose-fructose oxidoreductase from Zymomonas mobilis (Zm GFOR). We expressed the ORF CC1225 in the yeast Saccharomyces cerevisiae and used a yeast strain expressing the gene coding for Zm GFOR as a reference. Cell extracts of strains overexpressing CC1225 (renamed as Cc aaor) showed some Zm GFOR type of activity, producing D-gluconate and D-sorbitol when a mixture of D-glucose and D-fructose was used as substrate. However, the activity in Cc aaor expressing strain was >100-fold lower compared to strains expressing Zm gfor. Interestingly, C. crescentus AAOR was clearly more efficient than the Zm GFOR in converting in vitro a single sugar substrate D-xylose (10mM) to xylitol without an added cofactor, whereas this type of activity was very low with Zm GFOR. Furthermore, when cultured in the presence of D-xylose, the S. cerevisiae strain expressing Cc aaor produced nearly equal concentrations of D-xylonate and xylitol (12.5g D-xylonatel−1 and 11.5g D-xylitoll−1 from 26g D-xylosel−1), whereas the control strain and strain expressing Zm gfor produced only D-xylitol (5gl−1). Deletion of the gene encoding the major aldose reductase, Gre3p, did not affect xylitol production in the strain expressing Cc aaor, but decreased xylitol production in the strain expressing Zm gfor. In addition, expression of Cc aaor together with the D-xylonolactone lactonase encoding the gene xylC from C. crescentus slightly increased the final concentration and initial volumetric production rate of both D-xylonate and D-xylitol. These results suggest that C. crescentus AAOR is a novel type of oxidoreductase able to convert the single aldose substrate D-xylose to both its oxidized and reduced product. The Author(s), 2015 D-Xylonic acid nationallicence Xylitol nationallicence D-Xylose nationallicence Glucose-fructose oxidoreductase nationallicence GFOR nationallicence Wiebe Marilyn VTT, Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland aut Nygård Yvonne VTT, Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland aut Oja Merja VTT, Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland aut Andberg Martina VTT, Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland aut Ruohonen Laura VTT, Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland aut Koivula Anu VTT, Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland aut Penttilä Merja VTT, Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland aut Toivari Mervi VTT, Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9439-9447 0175-7598 99:22<9439 2015 99 253 https://doi.org/10.1007/s00253-015-6878-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6878-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wiebe Marilyn VTT, Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland aut NATIONALLICENCE 700 1- Nygård Yvonne VTT, Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland aut NATIONALLICENCE 700 1- Oja Merja VTT, Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland aut NATIONALLICENCE 700 1- Andberg Martina VTT, Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland aut NATIONALLICENCE 700 1- Ruohonen Laura VTT, Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland aut NATIONALLICENCE 700 1- Koivula Anu VTT, Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland aut NATIONALLICENCE 700 1- Penttilä Merja VTT, Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland aut NATIONALLICENCE 700 1- Toivari Mervi VTT, Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9439-9447 0175-7598 99:22<9439 2015 99 253