A novel chimeric flagellum fused with the multi-epitope vaccine CTB-UE prevents Helicobacter pylori -induced gastric cancer in a BALB/c mouse model
| LEADER | caa a22 4500 | ||
|---|---|---|---|
| 001 | 605501203 | ||
| 003 | CHVBK | ||
| 005 | 20210128100559.0 | ||
| 007 | cr unu---uuuuu | ||
| 008 | 210128e20151101xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-015-6705-z |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6705-z | ||
| 245 | 0 | 2 | |a A novel chimeric flagellum fused with the multi-epitope vaccine CTB-UE prevents Helicobacter pylori -induced gastric cancer in a BALB/c mouse model |h [Elektronische Daten] |c [Hui Song, Xiaobo Lv, Jue Yang, Wei Liu, Huan Yang, Tao Xi, Yingying Xing] |
| 520 | 3 | |a Helicobacter pylori (H. pylori) infection causes peptic ulcers, gastric adenocarcinoma, and mucosa-associated lymphoid tissue (MALT) lymphoma. The eradication of H. pylori might be an effective means of preventing gastric cancer. A dual-antigen epitope and dual-adjuvant vaccine called CTB-UE-CF (CCF) was constructed by combining a multi-epitope vaccine CTB-UE with a novel chimeric flagellum (CF) to simultaneously activate Toll-like receptor (TLR) 5-agonist activity and preserve the immunogenicity of H. pylori flagellum FlaA. The evaluation of efficacy to reduce H. pylori colonization was performed using BALB/c mice by oral immunization with a triple dose of this vaccine strain. Two weeks after the last immunization, mice were sacrificed to determine specific antibody levels and proinflammatory cytokine production. To determine the presence of H. pylori, we detected the number of H. pylori by real-time quantitative PCR (qPCR) and measured the urease activity in the gastric tissue. The results showed that the immunogenicity and mucosal immune responses of CCF performed significantly better than those of CTB-UE. This dual-antigen epitope and dual-adjuvant system might greatly contribute to the development of a safe and efficient therapeutic vaccine for humans against H. pylori infection. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Helicobacter pylori |2 nationallicence | |
| 690 | 7 | |a Toll-like receptor 5 |2 nationallicence | |
| 690 | 7 | |a Flagellum |2 nationallicence | |
| 690 | 7 | |a Vaccine |2 nationallicence | |
| 690 | 7 | |a Adjuvant |2 nationallicence | |
| 700 | 1 | |a Song |D Hui |u School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, China |4 aut | |
| 700 | 1 | |a Lv |D Xiaobo |u School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, China |4 aut | |
| 700 | 1 | |a Yang |D Jue |u School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, China |4 aut | |
| 700 | 1 | |a Liu |D Wei |u School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, China |4 aut | |
| 700 | 1 | |a Yang |D Huan |u School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, China |4 aut | |
| 700 | 1 | |a Xi |D Tao |u School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, China |4 aut | |
| 700 | 1 | |a Xing |D Yingying |u School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/22(2015-11-01), 9495-9502 |x 0175-7598 |q 99:22<9495 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6705-z |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6705-z |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Song |D Hui |u School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Lv |D Xiaobo |u School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yang |D Jue |u School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Liu |D Wei |u School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yang |D Huan |u School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Xi |D Tao |u School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Xing |D Yingying |u School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/22(2015-11-01), 9495-9502 |x 0175-7598 |q 99:22<9495 |1 2015 |2 99 |o 253 | ||