caa a22 4500 605501203 CHVBK 20210128100559.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00253-015-6705-z doi (NATIONALLICENCE)springer-10.1007/s00253-015-6705-z A novel chimeric flagellum fused with the multi-epitope vaccine CTB-UE prevents Helicobacter pylori -induced gastric cancer in a BALB/c mouse model [Elektronische Daten] [Hui Song, Xiaobo Lv, Jue Yang, Wei Liu, Huan Yang, Tao Xi, Yingying Xing] Helicobacter pylori (H. pylori) infection causes peptic ulcers, gastric adenocarcinoma, and mucosa-associated lymphoid tissue (MALT) lymphoma. The eradication of H. pylori might be an effective means of preventing gastric cancer. A dual-antigen epitope and dual-adjuvant vaccine called CTB-UE-CF (CCF) was constructed by combining a multi-epitope vaccine CTB-UE with a novel chimeric flagellum (CF) to simultaneously activate Toll-like receptor (TLR) 5-agonist activity and preserve the immunogenicity of H. pylori flagellum FlaA. The evaluation of efficacy to reduce H. pylori colonization was performed using BALB/c mice by oral immunization with a triple dose of this vaccine strain. Two weeks after the last immunization, mice were sacrificed to determine specific antibody levels and proinflammatory cytokine production. To determine the presence of H. pylori, we detected the number of H. pylori by real-time quantitative PCR (qPCR) and measured the urease activity in the gastric tissue. The results showed that the immunogenicity and mucosal immune responses of CCF performed significantly better than those of CTB-UE. This dual-antigen epitope and dual-adjuvant system might greatly contribute to the development of a safe and efficient therapeutic vaccine for humans against H. pylori infection. Springer-Verlag Berlin Heidelberg, 2015 Helicobacter pylori nationallicence Toll-like receptor 5 nationallicence Flagellum nationallicence Vaccine nationallicence Adjuvant nationallicence Song Hui School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, China aut Lv Xiaobo School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, China aut Yang Jue School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, China aut Liu Wei School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, China aut Yang Huan School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, China aut Xi Tao School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, China aut Xing Yingying School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9495-9502 0175-7598 99:22<9495 2015 99 253 https://doi.org/10.1007/s00253-015-6705-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6705-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Song Hui School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, China aut NATIONALLICENCE 700 1- Lv Xiaobo School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, China aut NATIONALLICENCE 700 1- Yang Jue School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, China aut NATIONALLICENCE 700 1- Liu Wei School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, China aut NATIONALLICENCE 700 1- Yang Huan School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, China aut NATIONALLICENCE 700 1- Xi Tao School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, China aut NATIONALLICENCE 700 1- Xing Yingying School of Life Science and Technology, China Pharmaceutical University, 210009, Nanjing, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/22(2015-11-01), 9495-9502 0175-7598 99:22<9495 2015 99 253