caa a22 4500 605501211 CHVBK 20210128100559.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00253-015-6663-5 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6663-5 Rapid detection of Yersinia pestis recombinant fraction 1 capsular antigen [Elektronische Daten] [Pei-Yi Tsui, Hui-Ping Tsai, Der-Jiang Chiao, Cheng-Che Liu, Rong-Hwa Shyu] Yersinia pestis, an infectious bacterium that is a causative agent of plague, a disease which has been shown to be one of the most feared in history and which has caused millions of deaths. The capsule-like fraction 1 (F1) antigen expressed by Y. pestis is a known specific marker for the identification of the bacteria; therefore, the detection of F1 is important for Y. pestis recognition. In this study, a rapid, sensitive, and specific technique, the lateral flow assay (LFA), was successfully developed to detect Y. pestis by the recombinant F1 antigen. The assay that utilized an anti-F1 polyclonal antibody (Pab) to identify the bacteria was based on a double-antibody sandwich format on a nitrocellulose membrane. With the LFA method, 50ng/ml of recombinant F1 protein and 105 CFU/mL of Y. pestis could be detected in less than 10min. This assay also showed no cross-reaction with other Yersinia spp. or with some selected capsule-producing Enterobacteriaceae strains. Furthermore, detection of Y. pestis in simulated samples has been evaluated. The detection sensitivity of Y. pestis in various matrices was 105 CFU/mL, which was identical to that in PBS buffer. The results obtained suggest that LFA is an excellent tool for detection of Y. pestis contamination in an environment and hence can be used to monitor plague diseases when they emerge. Springer-Verlag Berlin Heidelberg, 2015 Yersinia pestis nationallicence Capsule-like F1 antigen nationallicence Lateral flow assay nationallicence Immunogold detection nationallicence Mouse bioassay nationallicence Tsui Pei-Yi Institute of Preventive Medicine, National Defense Medical Center, P.O. Box 90048-700, Taipei, Taiwan, Republic of China aut Tsai Hui-Ping Institute of Preventive Medicine, National Defense Medical Center, P.O. Box 90048-700, Taipei, Taiwan, Republic of China aut Chiao Der-Jiang Institute of Preventive Medicine, National Defense Medical Center, P.O. Box 90048-700, Taipei, Taiwan, Republic of China aut Liu Cheng-Che Institute of Preventive Medicine, National Defense Medical Center, P.O. Box 90048-700, Taipei, Taiwan, Republic of China aut Shyu Rong-Hwa Institute of Preventive Medicine, National Defense Medical Center, P.O. Box 90048-700, Taipei, Taiwan, Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/18(2015-09-01), 7781-7789 0175-7598 99:18<7781 2015 99 253 https://doi.org/10.1007/s00253-015-6663-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6663-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tsui Pei-Yi Institute of Preventive Medicine, National Defense Medical Center, P.O. Box 90048-700, Taipei, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Tsai Hui-Ping Institute of Preventive Medicine, National Defense Medical Center, P.O. Box 90048-700, Taipei, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Chiao Der-Jiang Institute of Preventive Medicine, National Defense Medical Center, P.O. Box 90048-700, Taipei, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Liu Cheng-Che Institute of Preventive Medicine, National Defense Medical Center, P.O. Box 90048-700, Taipei, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Shyu Rong-Hwa Institute of Preventive Medicine, National Defense Medical Center, P.O. Box 90048-700, Taipei, Taiwan, Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/18(2015-09-01), 7781-7789 0175-7598 99:18<7781 2015 99 253