Enhanced rifamycin SV production by submerged fermentation using Amycolatopsis mediterranei
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6682-2 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6682-2 | ||
| 245 | 0 | 0 | |a Enhanced rifamycin SV production by submerged fermentation using Amycolatopsis mediterranei |h [Elektronische Daten] |c [M. Nagavalli, S. Ponamgi, V. Girijashankar, L. Rao] |
| 520 | 3 | |a Rifamycin is a broad-spectrum antimicrobial drug produced commercially by submerged fermentation where the yields are far less in comparison to its demand in human drug therapy. Addressing the need, sequential mutational strain improvement was carried using UV and EtBr that resulted in improved strain yielding rifamycin SV up to 4.32g/L. Further optimization of six important fermentation factors was followed which include temperature, agitation, inoculum level, period of fermentation, inorganic nitrogen source and amino acids. For the first time, we report a maximum yield of 5.32g/L of rifamycin SV. Among the amino acids, proline known for its slowest assimilation by Amycolatopsis mediterranei produced the highest improvement in antibiotic yields. Following mutational strain improvement and process optimization, a total of 3.8-fold increase in antibiotic titre was achieved. Following a conventional procedure of mutational strain improvement, highest yield of rifamycin SV was reported by optimizing submerged fermentation process. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Rifamycin SV |2 nationallicence | |
| 690 | 7 | |a Amycolatopsis mediterranei |2 nationallicence | |
| 690 | 7 | |a Mutational strain improvement |2 nationallicence | |
| 690 | 7 | |a Submerged fermentation optimization |2 nationallicence | |
| 690 | 7 | |a Proline |2 nationallicence | |
| 700 | 1 | |a Nagavalli |D M. |u Department of Microbiology, Osmania University, 500 007, Hyderabad, India |4 aut | |
| 700 | 1 | |a Ponamgi |D S. |u Center for Biotechnology, Jawaharlal Nehru Technological University, IST, Kukatpally, Hyderabad, India |4 aut | |
| 700 | 1 | |a Girijashankar |D V. |u Scientist C, Department of Science and Technology, DST, New Mehrauli, New Delhi, India |4 aut | |
| 700 | 1 | |a Rao |D L. |u Department of Microbiology, Osmania University, 500 007, Hyderabad, India |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/18(2015-09-01), 7505-7513 |x 0175-7598 |q 99:18<7505 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6682-2 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6682-2 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Nagavalli |D M. |u Department of Microbiology, Osmania University, 500 007, Hyderabad, India |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ponamgi |D S. |u Center for Biotechnology, Jawaharlal Nehru Technological University, IST, Kukatpally, Hyderabad, India |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Girijashankar |D V. |u Scientist C, Department of Science and Technology, DST, New Mehrauli, New Delhi, India |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Rao |D L. |u Department of Microbiology, Osmania University, 500 007, Hyderabad, India |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/18(2015-09-01), 7505-7513 |x 0175-7598 |q 99:18<7505 |1 2015 |2 99 |o 253 | ||